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Literature summary extracted from

  • Zhou, H.; Guan, W.; Zhou, M.; Shen, J.; Liu, X.; Wu, D.; Yin, X.; Xie, Y.
    Cloning and characterization of a gene encoding true D-cysteine desulfhydrase from Oryza sativa (2020), Plant Mol. Biol. Rep., 38, 95-113.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.15 expressed in Escherichia coli Rosetta (DE3) cells Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.15 S357E the mutation almost fully abolishes the enzyme activity Oryza sativa Japonica Group
4.4.1.15 S357E/T589L the mutations almost fully abolish the enzyme activity Oryza sativa Japonica Group
4.4.1.15 T389L the mutant retains 3.7% activity compared to the wild type enzyme Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.15 0.13
-
 D-cysteine at pH 8.5 and 37°C Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.15 chloroplast
-
 Oryza sativa Japonica Group 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.15 D-cysteine + H2O Oryza sativa Japonica Group
-
 sulfide + NH3 + pyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.15 Oryza sativa Japonica Group Q6ZHE5 cultivarWuyunjing 7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.15 Ni-NTA column chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.15 seedling
-
 Oryza sativa Japonica Group
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.15 0.015
-
 with L-cysteine as substrate, at pH 8.5 and 37°C Oryza sativa Japonica Group
4.4.1.15 43.36
-
 with D-cysteine as substrate, at pH 8.5 and 37°C Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.15 D-cysteine + H2O
-
 Oryza sativa Japonica Group sulfide + NH3 + pyruvate
-
 ?
4.4.1.15 D-cysteine + H2O 100% activity Oryza sativa Japonica Group sulfide + NH3 + pyruvate
-
 ?
4.4.1.15 L-cysteine + H2O 0.03% activity compared to D-cysteine Oryza sativa Japonica Group ?
-
 ?
4.4.1.15 additional information neither activities of 1-aminocyclopropane-1-carboxylate deaminase nor O-acetyl-L-serine (thiol) lyase can be detected from the recombinant protein Oryza sativa Japonica Group ?
-
-

Subunits

EC Number Subunits Comment Organism
4.4.1.15 ? x * 48000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
4.4.1.15 D-CDes
-
 Oryza sativa Japonica Group
4.4.1.15 DCD1
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.15 35
-
-
 Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.15 8.5
-
-
 Oryza sativa Japonica Group

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.4.1.15 Oryza sativa Japonica Group calculated from amino acid sequence
-
 7.1

General Information

EC Number General Information Comment Organism
4.4.1.15 physiological function the protein is a potentially important endogenous H2S producing enzyme in rice, which may play an important role in plant growth regulators and chemical stimuli Oryza sativa Japonica Group