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Literature summary extracted from

  • Liu, J.Y.; He, Z.D.; Leung, D.W.M.; Zeng, S.S.; Cui, L.L.; Peng, X.X.
    Molecular, biochemical and enzymatic characterization of photorespiratory 2-phosphoglycolate phosphatase (PGLP1) in rice (2022), Plant Biol., 24, 510-516.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.18 additional information PGLP activity in leaves of rice seedlings does not change significantly in daylight from 06:00 h to 21:00 h, regardless of the change in light intensity. Moving the rice seedlings from high CO2 (4000 ppm) to ambient air (400 ppm CO2) also does not result in any significant change in PGLP activity Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.18 gene Os04g0490800, cloning from rice leaves, DNA and aino acid sequence determination and analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged isozyme OsPGLP1 in rice leaf chloroplasts via transformation of protoplasts Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.18 additional information using CRISPR/Cas9 technology, a pglp1 rice mutant is generated in the Oryza sativa subsp. japonica Zhonghua 11 cultivar background. Mutation of PGLP1 results in suppressed PGLP activity and photorespiratory phenotype. The primary leaves of pglp1 mutant seedlings become chlorotic very soon after germination in ambient air and die in about 3 weeks. The mutant grows better in a CO2-enriched environment (4000 ppm CO2) compared to ambient air Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.18 additional information PGLP activity in leaves of rice seedlings does not change significantly in daylight from 06:00 h to 21:00 h, regardless of the change in light intensity. Moving the rice seedlings from high CO2 (4000 ppm) to ambient air (400 ppm CO2) also does not result in any significant change in PGLP activity Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.18 additional information
-
 additional information isozyme PGLP1 from rice leaves has typical Michaelis-Menten saturation kinetics for the substrate 2-phosphoglycolate Oryza sativa Japonica Group
3.1.3.18 0.272
-
 2-Phosphoglycolate pH 8.5, 22°C, recombinant enzyme Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.18 chloroplast
-
 Oryza sativa Japonica Group 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.18 Mg2+ required Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.18 65000
-
 native PAGE Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.18 2-Phosphoglycolate + H2O Oryza sativa Japonica Group
-
 Glycolate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.18 no activity by Oryza sativa chloroplast 2-phosphoglycolate phosphatase isozyme PGLP3
-
-
-
3.1.3.18 no activity by Oryza sativa cytoplasmic 2-phosphoglycolate phosphatase isozyme PGLP2
-
-
-
3.1.3.18 Oryza sativa Japonica Group Q7X7H3 cv. Zhonghua 11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.18 native enzyme from cell-free extract of leaf cells, by ultrafiltration, anion exchange chromatography, and again ultrafiltration Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.18 leaf
-
 Oryza sativa Japonica Group
-
3.1.3.18 leaf sheath
-
 Oryza sativa Japonica Group
-
3.1.3.18 additional information in rice leaves, levels of isozyme PGLP1 transcript are substantially higher than those of inactive isozymes PGLP2 and PGLP3, whereas in roots, levels of PGLP2 transcript are higher than those of PGLP1 and PGLP3 Oryza sativa Japonica Group
-
3.1.3.18 root
-
 Oryza sativa Japonica Group
-
3.1.3.18 seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.18 2-Phosphoglycolate + H2O
-
 Oryza sativa Japonica Group Glycolate + phosphate
-
 ?
3.1.3.18 additional information rice PGLP1 does not react with any other of the organic phosphate esters tested, i.e. glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), fructose-1,6-diphosphate (FBP), ribose-1, 5-diphosphate (RuBP) or 3-phosphoglyceric acid (3-PGA) Oryza sativa Japonica Group ?
-
-

Subunits

EC Number Subunits Comment Organism
3.1.3.18 homodimer 2 * 32000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
3.1.3.18 2-Phosphoglycolate phosphatase
-
 Oryza sativa Japonica Group
3.1.3.18 PGLP1
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.18 22
-
 assay at room temperature Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.18 7.5 10 broad optimum Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.1.3.18 malfunction no detectable PGLP activity in leaves of the OsPGLP1 mutant, which is non-viable in ambient air condition (400 ppm CO2) and high CO2 (4000 ppm) is unable to restore normal growth. In contrast, mutations of inactive isozymes PGLP2 or PGLP3 do not result in visible phenotypes and the leaf PGLP activities are also unaffected. Mutation of PGLP1 results in suppressed PGLP activity and photorespiratory phenotype. The primary leaves of pglp1 mutant seedlings become chlorotic very soon after germination in ambient air and die in about 3 weeks. The mutant grows better in a CO2-enriched environment (4000 ppm CO2) compared to ambient air Oryza sativa Japonica Group
3.1.3.18 metabolism under normal growth conditions, photosynthetic tissues, especially of C3 plants, will produce a large amount of 2-phosphoglycolate (2-PG) every day. If 2-PG is not dephosphorylated to glycolate, large amounts of C and P would be sequestered, and the accumulated 2-PG would inhibit activity of triose-phosphate isomerase, phosphofructokinase, and sedoheptulose 1,7-bisphosphate phosphatase. In addition, 2-PG is likely to suppress more enzymes of the Calvin cycle or enzymes involved in starch/sucrose biosynthesis/degradation, as well as transporters at the chloroplast membrane. Therefore, efficient 2-PG scavenging is vital for photosynthetic organisms. Phosphoglycolate phosphatase (PGLP) can catalyse the dephosphorylation of 2-PG to form glycolic acid, which produces 3-PGA through the photorespiration pathway and reenters the Calvin cycle Oryza sativa Japonica Group
3.1.3.18 physiological function the chloroplastidic isozyme PGLP1 is responsible for photorespiration in rice. Efficient 2-PG scavenging is vital for photosynthetic organisms. Phosphoglycolate phosphatase (PGLP) can catalyse the dephosphorylation of 2-PG to form glycolic acid, which produces 3-PGA through the photorespiration pathway and reenters the Calvin cycle Oryza sativa Japonica Group