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Literature summary extracted from

  • Wang, Y.H.; Liu, W.; Cheng, J.; Li, R.J.; Wen, J.; Mysore, K.S.; Xie, Z.P.; Reinhardt, D.; Staehelin, C.
    An extracellular beta-N-acetylhexosaminidase of Medicago truncatula hydrolyzes chitooligosaccharides and is involved in arbuscular mycorrhizal symbiosis but not required for nodulation (2023), New Phytol., 239, 1954-1973.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.52 expression in Pichia pastoris. Poor expression in Escherichia coli Medicago truncatula

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.52 D325A/E326A complete loss of activity Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Medicago truncatula
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Medicago truncatula
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O
-
 Medicago truncatula 4-nitrophenol + N-acetyl-beta-glucosamine
-
 ?
3.2.1.52 4-nitrophenyl-N-acetyl-beta-D-galactosaminide + H2O
-
 Medicago truncatula 4-nitrophenol + N-acetyl-beta-galactosamine
-
 ?
3.2.1.52 chitoheptaose + H2O
-
 Medicago truncatula chitohexaose + N-acetyl-beta-glucosamine
-
 ?
3.2.1.52 chitohexaose + H2O
-
 Medicago truncatula chitopentaose + N-acetyl-beta-glucosamine
-
 ?
3.2.1.52 chitopentaose + H2O
-
 Medicago truncatula chitotetraose + N-acetyl-beta-glucosamine
-
 ?
3.2.1.52 additional information no or very weak activity with 4-nitrophenyl-alpha-L-arabinofuranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, or 4-nitrophenyl-beta-D-galactopyranoside Medicago truncatula ?
-
-

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 68000, SDS-PAGE, recombinant protein Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 HEXO2
-
 Medicago truncatula
3.2.1.52 Medtr2g062560
-
 Medicago truncatula

Expression

EC Number Organism Comment Expression
3.2.1.52 Medicago truncatula HexO2 expression is upregulated during symbiosis up

General Information

EC Number General Information Comment Organism
3.2.1.52 physiological function HexO2 gene insertion mutants show decreased colonization by arbuscular mycorrhizal fungi while nodulation is not affected Medicago truncatula