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Literature summary extracted from
- Zeta-Carotene isomerase suppresses tillering in rice through the coordinated biosynthesis of strigolactone and abscisic acid (2020), Mol. Plant, 13, 1784-1801.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.2.1.12 | gene T20, quantitative real-time PCR enzyme expression analysis | Oryza sativa Indica Group |
| 5.2.1.12 | gene T20, quantitative real-time PCR enzyme expression analysis | Oryza sativa Japonica Group |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.2.1.12 | additional information | identification of a high-tillering mutant tillering20 (t20), which can be restored to the wild-type by treatment with the strigolactone (SL) analogue rac-GR24. T20 encodes a chloroplast z-carotene isomerase (Z-ISO), which is involved in the biosynthesis of carotenoids and their metabolites, SL and abscisic acid (ABA). The t20 mutant has reduced SL and ABA, phenotype, overview | Oryza sativa Japonica Group |
| 5.2.1.12 | additional information | identification of a high-tillering mutant tillering20 (t20), which can be restored to the wild-type by treatment with the strigolactone (SL) analogue rac-GR24. T20 encodes a chloroplast zeta-carotene isomerase (Z-ISO), which is involved in the biosynthesis of carotenoids and their metabolites, SL and abscisic acid (ABA). The t20 mutant has reduced SL and ABA, phenotype, overview | Oryza sativa Indica Group |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.2.1.12 | chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
| 5.2.1.12 | chloroplast | - |
Oryza sativa Indica Group | 9507 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.2.1.12 | 9,15,9'-tricis-zeta-carotene | Oryza sativa Japonica Group | - |
9,9'-dicis-zeta-carotene | - |
r |  |
| 5.2.1.12 | 9,15,9'-tricis-zeta-carotene | Oryza sativa Indica Group | - |
9,9'-dicis-zeta-carotene | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.2.1.12 | Oryza sativa Indica Group | B8BP90 | - |
- |
| 5.2.1.12 | Oryza sativa Japonica Group | Q2QT64 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.2.1.12 | seedling | - |
Oryza sativa Japonica Group | - |
| 5.2.1.12 | seedling | - |
Oryza sativa Indica Group | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.2.1.12 | 9,15,9'-tricis-zeta-carotene | - |
Oryza sativa Japonica Group | 9,9'-dicis-zeta-carotene | - |
r | |
| 5.2.1.12 | 9,15,9'-tricis-zeta-carotene | - |
Oryza sativa Indica Group | 9,9'-dicis-zeta-carotene | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.2.1.12 | T20 | - |
Oryza sativa Japonica Group |
| 5.2.1.12 | T20 | - |
Oryza sativa Indica Group |
| 5.2.1.12 | Z-ISO | - |
Oryza sativa Japonica Group |
| 5.2.1.12 | Z-ISO | - |
Oryza sativa Indica Group |
| 5.2.1.12 | zeta-carotene isomerase | - |
Oryza sativa Japonica Group |
| 5.2.1.12 | zeta-carotene isomerase | - |
Oryza sativa Indica Group |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 5.2.1.12 | Oryza sativa Japonica Group | rac-GR24 stimulates T20 expression and enhances all-trans beta-carotene biosynthesis. rac-GR24 also stimulates expression of Oryza sativa 9-cis-epoxycarotenoid dioxygenase 1 (OsNCED1) through induction of Oryza sativa homeobox12 (OsHOX12), promoting abscisic acid (ABA) biosynthesis in shoot base. But except for Z-ISO (T20), rac-GR24 has little effect on expression levels of carotenoid biosynthesis genes, including those encoding carotenoid isomerase (CRTISO), phytoene synthase1, 2, and 3 (PSY1, PSY2, and PSY3), phytoene desaturase (PDS), zeta-carotene desaturase (ZDS), and lycopene-beta-cyclase (LCYB). Stringolactones (SLs) also induce expression of Z-ISO(T20) | up |
| 5.2.1.12 | Oryza sativa Indica Group | rac-GR24 stimulates T20 expression and enhances all-trans beta-carotene biosynthesis. rac-GR24 also stimulates expression of Oryza sativa 9-cis-epoxycarotenoid dioxygenase 1 (OsNCED1) through induction of Oryza sativa homeobox12 (OsHOX12), promoting abscisic acid (ABA) biosynthesis in shoot base. But except for Z-ISO (T20), rac-GR24 has little effect on expression levels of carotenoid biosynthesis genes, including those encoding carotenoid isomerase (CRTISO), phytoene synthase1, 2, and 3 (PSY1, PSY2, and PSY3), phytoene desaturase (PDS), zeta-carotene desaturase (ZDS), and lycopene-beta-cyclase (LCYB). Stringolactones (SLs) also induce expression of Z-ISO (T20) | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.2.1.12 | malfunction | endogenous strigolactone (SL) and abscisic acid (ABA) levels are both reduced in the high-tillering mutant tillering20 (t20), the mutant can be restored to the wild-type by treatment with the strigolactone (SL) analogue rac-GR24. Abscisic acid (ABA) treatment significantly represses strigolactone (SL) biosynthesis, and the ABA biosynthetic mutants displays elevated SL biosynthesis. ABA treatment reduces the number of basal tillers in both t20 and wild-type plants. While ABA-deficient mutants aba1 and aba2 had the same number of basal tillers as wild type, they had more unproductive upper tillers at maturity | Oryza sativa Japonica Group |
| 5.2.1.12 | malfunction | endogenous strigolactone (SL) and abscisic acid (ABA) levels are both reduced in the high-tillering mutant tillering20 (t20), the mutant can be restored to the wild-type by treatment with the strigolactone (SL) analogue rac-GR24. Abscisic acid (ABA) treatment significantly represses strigolactone (SL) biosynthesis, and the ABA biosynthetic mutants displays elevated SL biosynthesis. ABA treatment reduces the number of basal tillers in both t20 and wild-type plants. While ABA-deficient mutants aba1 and aba2 had the same number of basal tillers as wild type, they had more unproductive upper tillers at maturity | Oryza sativa Indica Group |
| 5.2.1.12 | physiological function | gene T20 encodes zeta-carotene isomerase (Z-ISO), which catalyzes a key step in carotenoid biosynthesis. Z-ISO (T20) catalyzes the isomerization of 9,15,9'-tri-cis-zeta-carotene to 9,9'-di-cis-zeta-carotene, which is converted to all-trans beta-carotene through several subsequent steps. Zeta-Carotene isomerase suppresses tillering in rice through the coordinated biosynthesis of strigolactone and abscisic acid. The chloroplast zeta-carotene isomerase (Z-ISO) is involved in the biosynthesis of carotenoids and their metabolites, strigolactone (SL) and abscisic acid (ABA) | Oryza sativa Japonica Group |
| 5.2.1.12 | physiological function | gene T20 encodes zeta-carotene isomerase (Z-ISO), which catalyzes a key step in carotenoid biosynthesis. Z-ISO (T20) catalyzes the isomerization of 9,15,9'-tri-cis-z-carotene to 9,9'-di-cis-zeta-carotene, which is converted to all-trans beta-carotene through several subsequent steps. Zeta-Carotene isomerase suppresses tillering in rice through the coordinated biosynthesis of strigolactone and abscisic acid. The chloroplast zeta-carotene isomerase (Z-ISO) is involved in the biosynthesis of carotenoids and their metabolites, strigolactone (SL) and abscisic acid (ABA) | Oryza sativa Indica Group |
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