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Literature summary extracted from

  • Shukal, S.; Chen, X.; Zhang, C.
    Systematic engineering for high-yield production of viridiflorol and amorphadiene in auxotrophic Escherichia coli (2019), Metab. Eng., 55, 170-178.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.B79 synthesis production of viridiflorol in recombinant Escherichia coli via modification of the mevalonate and viridiflorol pathways at both transcriptional and translational levels. The best strain achieves 25.7 g/l and a yield of 0.22 g viridiflorol per g glucose in 2.5 days Cyclocybe aegerita

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.B79 homology modeling of structure and docking of Mg2+ and diphosphate Cyclocybe aegerita

Protein Variants

EC Number Protein Variants Comment Organism
4.2.3.B79 G227C/E267S/V314Y mutations improve viridiflorol production by 52% Cyclocybe aegerita
4.2.3.B79 G227C/V314Y mutations improve viridiflorol production by 38% Cyclocybe aegerita
4.2.3.B79 G227C/V314Y/M318V mutations reduce viridiflorol production by 90% Cyclocybe aegerita
4.2.3.B79 L174F mutation reduces viridiflorol production by more than 95% Cyclocybe aegerita
4.2.3.B79 L174I mutation reduces viridiflorol production by more than 95% Cyclocybe aegerita
4.2.3.B79 L174M mutation reduces viridiflorol production by more than 95%. L174 is positioned in vicinity of magnesium binding motif DEYTD Cyclocybe aegerita
4.2.3.B79 L174T mutation reduces viridiflorol production by more than 95% Cyclocybe aegerita
4.2.3.B79 L174V mutation reduces viridiflorol production by more than 95% Cyclocybe aegerita
4.2.3.B79 L174Y mutation reduces viridiflorol production by more than 95% Cyclocybe aegerita
4.2.3.B79 additional information removal of amino acids 2-85 from the N-terminus, i.e. the N-terminal signal peptide, of the wild-type and the double mutant G227C/V314Y markedly increases the viridiflorol titres and specific yields by about 200% and 100% for the wild-type and the mutant, respectively Cyclocybe aegerita
4.2.3.B79 V314Y mutation reduces viridiflorol production by more than 50% Cyclocybe aegerita

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.B79 Mg2+
-
 Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.B79 Cyclocybe aegerita A0A5Q0QSI8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.B79 (2E,6E)-farnesyl diphosphate + H2O
-
 Cyclocybe aegerita viridiflorol + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.B79 Agr5
-
 Cyclocybe aegerita