Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhao, W.; Geng, H.; Dan, Z.; Zeng, Y.; Wang, M.; Xu, W.; Hu, Z.; Huang, W.
    The alpha subunit of mitochondrial processing peptidase participated in fertility restoration in Honglian-CMS rice (2023), Int. J. Mol. Sci., 24, 5442.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.64 cloning of MPPA from wild-type Rf6-NIL, derived from repeated backcrossing of 9311 to (Yuetai A, HL-CMS line), DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged MPPA and recombinant expression of MBP-tagged MPPA in Escherichia coli strain BL21(DE3) Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.64 additional information the mppa+/- and mppa-/- mutant plants are generated via the CRISPR/Cas9 system Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.64 Zn2+ a zinc metallopeptidase Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.64 additional information Oryza sativa Japonica Group MPPA interacts with RF6 in vivo and in vitro ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.64 Oryza sativa Japonica Group B7EBD6 alpha subunit; cv. Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.64 recombinant His-tagged MPPA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant MBP-tagged MPPA from Escherichia coli strain BL21(DE3) by amylose affinity chromatography Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.64 additional information MPPA interacts with RF6 in vivo and in vitro Oryza sativa Japonica Group ?
-
-

Synonyms

EC Number Synonyms Comment Organism
3.4.24.64 mitochondrial processing peptidase
-
 Oryza sativa Japonica Group
3.4.24.64 mitochondrial processing peptidase alpha subunit
-
 Oryza sativa Japonica Group
3.4.24.64 MPPA
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.4.24.64 malfunction the alpha subunit of mitochondrial processing peptidase participated in fertility restoration in Honglian-cytoplasmic male sterility (CMS) rice. The alpha subunit of mitochondrial processing peptidase (MPPA) is involved in the fertility restoration process in Honglian-CMS rice. In rice, three major types of cytoplasmic male sterility (CMS) have been widely acknowledged: the WA (wild abortive)-CMS is sporophytic CMS caused by WA352, and the Rf3 and Rf4 are the two Rf proteins encoding genes responsible for eliminating the influence of WA352 on fertility. The HL (Honglian)-CMS is gametophytic CMS caused by atp6-orfH79, of which sterility can be rescued by Rf5 and Rf6. The BT (ChinsurahBoro II)-CMS is gametophytic CMS caused by atp6-orf79, and the Rf1a, Rf1b, and Rf6 proteins contribute to rescuing BT-CMS. The Rf proteins alone are not sufficient for rescuing the sterile phenotype in many cases, they work with other proteins for precise cleavage of the CMS-associated transcripts in mitochondria to rescue sterility, suggesting the complexity of the mechanisms in fertility restoration. Loss-of-function of MPPA results in a defect in pollen fertility, the mppa+/- heterozygotes show semi-sterility phenotype and the accumulation of CMS-associated protein ORFH79, exhibiting restrained processing of the CMS-associated atp6-OrfH79 in the mutant plant. Sterile phenotype, overview Oryza sativa Japonica Group
3.4.24.64 physiological function the alpha subunit of mitochondrial processing peptidase (MPPA) is a mitochondrial localized protein and interacted with the RF6 protein encoded by the Rf6. MPPA indirectly interacted with hexokinase 6, namely another partner of RF6, to form a protein complex with the same molecular weight as the mitochondrial F1F0-ATP synthase in processing the CMS transcript Oryza sativa Japonica Group