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Literature summary extracted from

  • Lampitella, E.; Landi, N.; Oliva, R.; Ragucci, S.; Petraccone, L.; Berisio, R.; Di Maro, A.; Del Vecchio, P.
    Conformational stability of ageritin, a metal binding ribotoxin-like protein of fungal origin (2022), Int. J. Biol. Macromol., 221, 1012-1021.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.6.1.23 ageritin thermodynamic stability is related to Zn2+ ion binding at Cys18 Cyclocybe aegerita

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.23 additional information modification of the enzyme by alkylating the single Cys18 residue, which is predicted to bind Zn2+. S-pyridylethylated ageritin preserves both structure and activity, but S-pyridylethylated ageritin has low stability compared to the native protein Cyclocybe aegerita

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.23 additional information
-
 additional information kinetics and thermodynamics of wild-type and modified S-pyridylethylated ageritin Cyclocybe aegerita

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.23 Zn2+ required, ageritin thermodynamic stability is related to Zn2+ ion binding at Cys18 Cyclocybe aegerita

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.23 rRNA + H2O Cyclocybe aegerita
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.23 Cyclocybe aegerita C0HLG3 Agrocybe aegerita
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.23 rRNA + H2O
-
 Cyclocybe aegerita ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.23 ageritin
-
 Cyclocybe aegerita
4.6.1.23 ribotoxin-like protein
-
 Cyclocybe aegerita

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.23 25
-
 assay at Cyclocybe aegerita

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.6.1.23 78
-
 high protein thermal stability at all pHs with a denaturation temperature of 78°C Cyclocybe aegerita

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.23 5
-
 assay at Cyclocybe aegerita

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.6.1.23 4 7.4 ageritin is highly stable within this pH range Cyclocybe aegerita

General Information

EC Number General Information Comment Organism
4.6.1.23 physiological function ageritin is a specific ribonuclease with major rRNA as target Cyclocybe aegerita