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Literature summary extracted from

  • Maeda, M.; Okamoto, N.; Araki, N.; Kimura, Y.
    Purification, characterization, and gene expression of rice endo-beta-N-acetylglucosaminidase, Endo-Os (2021), Front. Plant Sci., 12, 647684.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.96 expression in Escherichia coli Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.96 Cu2+ 6 mM, 90% inhibition Oryza sativa Japonica Group
3.2.1.96 Mn2+ 6 mM, 46% inhibition Oryza sativa Japonica Group
3.2.1.96 additional information EDTA (10 mM) has no effect on the activity Oryza sativa Japonica Group
3.2.1.96 Urea 1 M, 70% inhibition Oryza sativa Japonica Group
3.2.1.96 Zn2+ 6 mM, 90% inhibition Oryza sativa Japonica Group

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 70000
-
 SDS-PAGE Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Oryza sativa Japonica Group Q5W6R1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96
-
 Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 high-mannose type N-glycan + H2O the enzyme shows high activity for high-mannose type N-glycans bearing the Manalpha1-2Manalpha1-3Manbeta1 unit Oryza sativa Japonica Group ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.96 ? x * 70000, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
3.2.1.96 endo-beta-GlcNAc-ase
-
 Oryza sativa Japonica Group
3.2.1.96 Endo-Os
-
 Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.96 50
-
-
 Oryza sativa Japonica Group

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.96 20 60 20°C: about 60% of maximal activity, 60°C: about 65% of maximal activity Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 6.5
-
-
 Oryza sativa Japonica Group

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.96 4.5 7 pH 4.5: about 50% of maximal activity, pH 7.0: about 45% of maximal activity Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
3.2.1.96 physiological function the enzyme plays a critical role in the production of high-mannose type free N-glycans in the cytosol. In the endoplasmic reticulum-associated degradation system of plant and animal cells, high-mannose type free N-glycans are produced from misfolded glycoproteins prior to proteasomal degradation. Two enzymes, cytosolic peptide:N-glycanase (cPNGase) and endo-beta-N-acetylglucosaminidase (endo-b-GlcNAc-ase), are involved in the deglycosylation Oryza sativa Japonica Group