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Literature summary extracted from

  • Witzel, K.; Abu Risha, M.; Albers, P.; Boernke, F.; Hanschen, F.S.
    Identification and characterization of three epithiospecifier protein isoforms in Brassica oleracea (2019), Front. Plant Sci., 10, 1552.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.6 ESP protein isozymes 1 to 3, ESP1-3, DNA and amino acid sequence determination and analysis, genotyping and expression profiles. Quantitative real-time PCR enzyme expression analysis, BoESP isoforms exhibit a differential expression pattern. Recombinant expression of ESP isozymes 1-3 in Arabidopsis thaliana strain Hi-0, an ecotype with a GLS pattern that is characterized by high allyl GLS content and barely detectable own ESP activity Brassica oleracea var. oleracea
4.8.1.6 ESP protein, DNA and amino acid sequence determination and analysis, genotyping Brassica oleracea var. gongylodes
4.8.1.6 ESP protein, DNA and amino acid sequence determination and analysis, genotyping Brassica oleracea var. italica
4.8.1.6 ESP protein, DNA and amino acid sequence determination and analysis, genotyping. BoESP isoforms exhibit a differential expression pattern Brassica oleracea var. capitata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.8.1.6 Fe2+ best at 0.2 mM Brassica oleracea var. capitata
4.8.1.6 Fe2+ best at 0.2 mM Brassica oleracea var. gongylodes
4.8.1.6 Fe2+ best at 0.2 mM Brassica oleracea var. italica
4.8.1.6 Fe2+ best at 0.2 mM Brassica oleracea var. oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.8.1.6 additional information Brassica oleracea var. capitata ESP activity is detected via plant tissue autolysis assay ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.6 Brassica oleracea var. capitata
-
 f. alba cv. Jetma, white cabbage
-
4.8.1.6 Brassica oleracea var. capitata
-
 f. rubra cv. Integro, red cabbage
-
4.8.1.6 Brassica oleracea var. gongylodes
-
 cv. Kolibri, kohlrabi
-
4.8.1.6 Brassica oleracea var. italica Q4TU02 cv. Ironman, broccoli
-
4.8.1.6 Brassica oleracea var. oleracea A0A0D3CQU9
-
-
4.8.1.6 Brassica oleracea var. oleracea A0A0D3D8Y4
-
-
4.8.1.6 Brassica oleracea var. oleracea A0A0D3AU36
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.8.1.6 additional information roots possess a lower ESP activity as compared to shoots. BoESP1-3 isoforms exhibit differential expression patterns, overview Brassica oleracea var. capitata
-
4.8.1.6 additional information roots possess a lower ESP activity as compared to shoots. BoESP1-3 isoforms exhibit differential expression patterns, overview Brassica oleracea var. gongylodes
-
4.8.1.6 additional information roots possess a lower ESP activity as compared to shoots. BoESP1-3 isoforms exhibit differential expression patterns, overview Brassica oleracea var. italica
-
4.8.1.6 additional information BoESP1-3 isoforms exhibit differential expression patterns, overview Brassica oleracea var. oleracea
-
4.8.1.6 root
-
 Brassica oleracea var. capitata
-
4.8.1.6 root
-
 Brassica oleracea var. gongylodes
-
4.8.1.6 root low ESP activity Brassica oleracea var. capitata
-
4.8.1.6 root
-
 Brassica oleracea var. italica
-
4.8.1.6 root
-
 Brassica oleracea var. oleracea
-
4.8.1.6 shoot high ESP activity Brassica oleracea var. capitata
-
4.8.1.6 shoot high ESP activity Brassica oleracea var. gongylodes
-
4.8.1.6 shoot low ESP activity Brassica oleracea var. capitata
-
4.8.1.6 shoot high ESP activity Brassica oleracea var. italica
-
4.8.1.6 shoot
-
 Brassica oleracea var. oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.6 1-cyano- 3,4-epithiobutane CETB Brassica oleracea var. oleracea ?
-
 ?
4.8.1.6 1-cyano-2,3-epithiopropane CETP Brassica oleracea var. capitata ?
-
 ?
4.8.1.6 1-cyano-2,3-epithiopropane CETP Brassica oleracea var. gongylodes ?
-
 ?
4.8.1.6 1-cyano-2,3-epithiopropane CETP Brassica oleracea var. italica ?
-
 ?
4.8.1.6 1-cyano-2,3-epithiopropane CETP Brassica oleracea var. oleracea ?
-
 ?
4.8.1.6 1-cyano-2-hydroxy-3,4-epithiobutane CHETB Brassica oleracea var. capitata ?
-
 ?
4.8.1.6 1-cyano-2-hydroxy-3,4-epithiobutane CHETB Brassica oleracea var. gongylodes ?
-
 ?
4.8.1.6 1-cyano-2-hydroxy-3,4-epithiobutane CHETB Brassica oleracea var. italica ?
-
 ?
4.8.1.6 1-cyano-2-hydroxy-3,4-epithiobutane CHETB Brassica oleracea var. oleracea ?
-
 ?
4.8.1.6 1-cyano-3,4-epithiobutane CETB Brassica oleracea var. capitata ?
-
 ?
4.8.1.6 1-cyano-3,4-epithiobutane CETB Brassica oleracea var. gongylodes ?
-
 ?
4.8.1.6 1-cyano-3,4-epithiobutane CETB Brassica oleracea var. italica ?
-
 ?
4.8.1.6 1-cyano-3,4-epithiobutane CETB Brassica oleracea var. oleracea ?
-
 ?
4.8.1.6 additional information ESP activity is detected via plant tissue autolysis assay Brassica oleracea var. capitata ?
-
-
4.8.1.6 additional information the enzyme is active on allyl glucosinolates (GLS). Isozyme BoESP3 shows the highest ESP activity, and isozyme BoESP2 (by tendency for allyl GLS hydrolysis) the lowest ESP activity. Substrate specificity, overview Brassica oleracea var. capitata ?
-
-
4.8.1.6 additional information the enzyme is active on allyl glucosinolates (GLS). Isozyme BoESP3 shows the highest ESP activity, and isozyme BoESP2 (by tendency for allyl GLS hydrolysis) the lowest ESP activity. Substrate specificity, overview Brassica oleracea var. gongylodes ?
-
-
4.8.1.6 additional information the enzyme is active on allyl glucosinolates (GLS). Isozyme BoESP3 shows the highest ESP activity, and isozyme BoESP2 (by tendency for allyl GLS hydrolysis) the lowest ESP activity. Substrate specificity, overview Brassica oleracea var. italica ?
-
-
4.8.1.6 additional information the enzyme is active on allyl glucosinolates (GLS). Isozyme BoESP3 shows the highest ESP activity, and isozyme BoESP2 (by tendency for allyl GLS hydrolysis) the lowest ESP activity. Substrate specificity, overview Brassica oleracea var. oleracea ?
-
-
4.8.1.6 sulfopharan (RS)-1-isothiocyanato-4-methylsulfinylbutane Brassica oleracea var. capitata ?
-
 ?
4.8.1.6 sulfopharan (RS)-1-isothiocyanato-4-methylsulfinylbutane Brassica oleracea var. gongylodes ?
-
 ?
4.8.1.6 sulfopharan (RS)-1-isothiocyanato-4-methylsulfinylbutane Brassica oleracea var. italica ?
-
 ?
4.8.1.6 sulfopharan (RS)-1-isothiocyanato-4-methylsulfinylbutane Brassica oleracea var. oleracea ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.8.1.6 BoESP1
-
 Brassica oleracea var. capitata
4.8.1.6 BoESP1
-
 Brassica oleracea var. oleracea
4.8.1.6 BoESP2
-
 Brassica oleracea var. capitata
4.8.1.6 BoESP2
-
 Brassica oleracea var. oleracea
4.8.1.6 BoESP3
-
 Brassica oleracea var. capitata
4.8.1.6 BoESP3
-
 Brassica oleracea var. oleracea
4.8.1.6 ESP
-
 Brassica oleracea var. capitata
4.8.1.6 ESP
-
 Brassica oleracea var. gongylodes
4.8.1.6 ESP
-
 Brassica oleracea var. italica
4.8.1.6 ESP
-
 Brassica oleracea var. oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.8.1.6 22
-
 assay at room temperature Brassica oleracea var. capitata
4.8.1.6 22
-
 assay at room temperature Brassica oleracea var. gongylodes
4.8.1.6 22
-
 assay at room temperature Brassica oleracea var. italica
4.8.1.6 22
-
 assay at room temperature Brassica oleracea var. oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8.1.6 5.5
-
 assay at Brassica oleracea var. capitata
4.8.1.6 5.5
-
 assay at Brassica oleracea var. gongylodes
4.8.1.6 5.5
-
 assay at Brassica oleracea var. italica
4.8.1.6 5.5
-
 assay at Brassica oleracea var. oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.8.1.6 4 7 while BoESP3 activity is high at all pH values, both BoESP1 and BoESP2 are affected. Being at optimal pH from pH 6.0 to pH 7.0, their activity decreases with decreasing pH value. This effect is stronger for BoESP2 compared to BoESP1, especially at pH 4.0 Brassica oleracea var. oleracea

General Information

EC Number General Information Comment Organism
4.8.1.6 evolution comparisons and protein profiling of ESP proteins from four different Brassica oleraceae varieties, crops sprouts and roots, with respect to GLS profile, GLS hydrolysis products, ESP activity, and transcript profiling. Every variety contains 3 isozymes, ESP1-3. Both, broccoli and kohlrabi are rich in methylsulfanyl- and methylsulfinylalkyl GLS, but contain no alkenyl GLS Brassica oleracea var. capitata
4.8.1.6 evolution comparisons and protein profiling of ESP proteins from four different Brassica oleraceae varieties, crops sprouts and roots, with respect to GLS profile, GLS hydrolysis products, ESP activity, and transcript profiling Brassica oleracea var. gongylodes
4.8.1.6 evolution comparisons and protein profiling of ESP proteins from four different Brassica oleraceae varieties, crops sprouts and roots, with respect to GLS profile, GLS hydrolysis products, ESP activity, and transcript profiling Brassica oleracea var. capitata
4.8.1.6 evolution comparisons and protein profiling of ESP proteins from four different Brassica oleraceae varieties, crops sprouts and roots, with respect to GLS profile, GLS hydrolysis products, ESP activity, and transcript profiling Brassica oleracea var. italica