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Literature summary extracted from

  • Arias-Gaguancela, O.; Herrell, E.; Chapman, K.D.
    Ex vivo lipidomics reveal monoacylglycerols as substrates for a fatty acid amide hydrolase in the legume Medicago truncatula (2024), FEBS Lett., 598, 1839-1854.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.99 expressed in Escherichia coli BL21(DE3) cells Medicago truncatula

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.99 ethanolamine product inhibition Medicago truncatula
3.5.1.99 glycerol product inhibition Medicago truncatula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.99 0.02868
-
 palmitoylethanolamide at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 0.05416
-
 sn-2-palmitoylglycerol at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 0.1867
-
 sn-1-palmitoyl-rac-glycerol at pH 9.0 and 30°C Medicago truncatula

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.99 Medicago truncatula
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.99 Ni-NTA agarose resin column chromatography and Superdex 200 gel filtration Medicago truncatula

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.99 palmitoylethanolamide + H2O
-
 Medicago truncatula palmitic acid + ethanolamine
-
 ?
3.5.1.99 sn-1-palmitoyl-rac-glycerol + H2O
-
 Medicago truncatula palmitic acid + glycerol
-
 ?
3.5.1.99 sn-2-palmitoylglycerol + H2O
-
 Medicago truncatula palmitic acid + glycerol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.99 ? x * 70000, SDS-PAGE Medicago truncatula

Synonyms

EC Number Synonyms Comment Organism
3.5.1.99 FAAH1
-
 Medicago truncatula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.99 0.17
-
 palmitoylethanolamide at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 0.7 1 sn-2-palmitoylglycerol at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 1.2
-
 sn-1-palmitoyl-rac-glycerol at pH 9.0 and 30°C Medicago truncatula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.99 5.79
-
 palmitoylethanolamide at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 6.43
-
 sn-1-palmitoyl-rac-glycerol at pH 9.0 and 30°C Medicago truncatula
3.5.1.99 13.1
-
 sn-2-palmitoylglycerol at pH 9.0 and 30°C Medicago truncatula