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Literature summary extracted from

  • Wang, X.; Pan, H.; Sagurthi, S.; Paris, V.; Zhuo, C.; Dixon, R.A.
    The protein conformational basis of isoflavone biosynthesis (2022), Commun. Biol., 5, 1249.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.87 expression in Escherichia coli Medicago truncatula
4.2.1.105 expression in Escherichia coli Pueraria montana var. lobata

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.14.87 hanging drops by the vapor diffusion method, the structure reveals the active site architecture and putative catalytic residues for the dual dehydratase and carboxylesterase activities Medicago truncatula
4.2.1.105 hanging drops by the vapor diffusion method, the structure reveals a unique cytochrome P450 conformation and heme and substrate binding mode that facilitate the coupled aryl-ring migration and hydroxylation reactions Pueraria montana var. lobata

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.105 D269A n carboxylesterase activity and 3% dehydration activity as compared to wild-type enzyme Pueraria montana var. lobata
4.2.1.105 E89A 23% carboxylesterase activity as compared to wild-type enzyme and no dehydration activity Pueraria montana var. lobata
4.2.1.105 E89Q 50 % carboxylesterase activity as compared to wild-type enzyme and nodehydration activity Pueraria montana var. lobata
4.2.1.105 H301A no carboxylesterase activity and no dehydration activity Pueraria montana var. lobata
4.2.1.105 S169A 52% carboxylesterase activity as compared to wild-type enzyme and no dehydration activity Pueraria montana var. lobata
4.2.1.105 T168A 3% carboxylesterase activity and 1% dehydration activity as compared to wild-type enzyme Pueraria montana var. lobata

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.87 Medicago truncatula Q49BZ0
-
-
4.2.1.105 Pueraria montana var. lobata E9M5G1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.87
-
 Medicago truncatula
4.2.1.105
-
 Pueraria montana var. lobata

Synonyms

EC Number Synonyms Comment Organism
1.14.14.87 2-HIS
-
 Medicago truncatula
4.2.1.105 2-HID
-
 Pueraria montana var. lobata