Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nawaz, G.; Kang, H.
    Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation (2019), BMC Plant Biol., 19, 17.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.6.2.7 recombinant expression of GFP-tagged enzyme OsRH58 in Nicotiana tabacum leaves, recombinant expression of the enzyme in Arabidopsis thaliana plants Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
5.6.2.7 additional information transgenic Arabidopsis thaliana plants show upregulation of the recombinant expression of the enzyme by salt, drought, or heat stress, whereas its expression is decreased by cold, UV, or ABA treatment, phenotypes, overview. OsRH58 increases the growth and seed yield of Arabidopsis plants under normal conditions, and OsRH58 has positive effects on seed germination of Arabidopsis under salinity or drought stress, compared to wild-type enzyme. Enzyme OsRH58 confers salt or drought tolerance but not cold tolerance. Approximately 85% of transgenic seedlings survive after recovery from 4 days of drought stress, whereas approx. 50% of the wild-type seedlings survive. Given 300 mM mannitol treatment, approximately 25% and 80% of transgenic seeds germinate on the second and third day, respectively, whereas only 3% and 40% of wild-type seeds germinate on the same days. In contrast, the wild-type and transgenic seeds germinate without noticeable differences under cold stress or ABA treatment, detailed overview Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.7 chloroplast
-
 Oryza sativa Japonica Group 9507
-
5.6.2.7 additional information enzyme subcellular localization analysis. The enzyme contains a 60 amino acid (aa)-long chloroplast transit peptide (cTP) at the N-terminus Oryza sativa Japonica Group
-
-

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.7 Oryza sativa Japonica Group Q0JFN7
-
-

Subunits

EC Number Subunits Comment Organism
5.6.2.7 additional information enzyme domain structure, overview Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
5.6.2.7 chloroplast-localized DEAD-box RH
-
 Oryza sativa Japonica Group
5.6.2.7 DEAD-box RNA helicase
-
 Oryza sativa Japonica Group
5.6.2.7 Os01g73900 locus name Oryza sativa Japonica Group
5.6.2.7 OsRH58
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
5.6.2.7 Oryza sativa Japonica Group expression of OsRH58 in rice is decreased by cold, UV, or ABA treatment, detailed overview down
5.6.2.7 Oryza sativa Japonica Group expression of OsRH58 in rice is upregulated by salt, drought, or heat stress up

General Information

EC Number General Information Comment Organism
5.6.2.7 additional information the enzyme contains the Asp-Glu-Ala-Asp (DEAD) motif Oryza sativa Japonica Group
5.6.2.7 physiological function DEAD-box RHs are cellular molecules important for plant growth and fitness in harsh environments, overview. The chloroplast-transported DEAD-box RNA helicase OsRH58 possessing RNA chaperone activity confers stress tolerance by increasing translation of chloroplast mRNAs. The enzyme can complement the Escherichia coli mutant BX04 cells that are sensitive to cold shock because of the lack of RNA chaperones Oryza sativa Japonica Group