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Literature summary extracted from

  • Toporkova, Y.Y.; Gorina, S.S.; Bessolitsyna, E.K.; Smirnova, E.O.; Fatykhova, V.S.; Bruehlmann, F.; Ilyina, T.M.; Mukhtarova, L.S.; Grechkin, A.N.
    Double function hydroperoxide lyases/epoxyalcohol synthases (CYP74C) of higher plants identification and conversion into allene oxide synthases by site-directed mutagenesis (2018), Biochim. Biophys. Acta Mol. Cell Biol. Lipids, 1863, 369-378.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
5.3.99.6 A287G the variant is highly specific towards 9-HPOT Cucumis sativus
5.3.99.6 L93F/G283A the mutant exhibits higher turnover rates than those of the wild type enzyme with all hydroperoxide substrates tested Cucumis sativus
5.3.99.6 L97F the mutant converts 9-HPOD into the alpha-ketol, 9-oxononanoic acid and 9,10-epoxy-11-hydroxy-12-octadecenoic acid at a ratio 28:14:58. No allene oxide synthase products are detected after incubations of 9-HPOT, 13-HPOD and 13-HPOT Medicago truncatula
5.3.99.6 L98F/A287G the mutant exhibits higher turnover rates than those of the wild type enzyme with all hydroperoxide substrates tested Cucumis sativus

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.6 Cucumis sativus U5YTX9
-
-
5.3.99.6 Medicago truncatula Q7X9B4
-
-

Synonyms

EC Number Synonyms Comment Organism
5.3.99.6 9-AOS
-
 Cucumis sativus
5.3.99.6 9-AOS
-
 Medicago truncatula
5.3.99.6 CYP74C31
-
 Cucumis sativus
5.3.99.6 CYP74C31
-
 Medicago truncatula