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Literature summary extracted from

  • Zhang, C.; Chen, X.; Orban, A.; Shukal, S.; Birk, F.; Too, H.; Ruehl, M.
    Agrocybe aegerita serves as a gateway for identifying sesquiterpene biosynthetic enzymes in higher fungi (2020), ACS Chem. Biol., 15, 1268-1277.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.B79 expression in Escherichia coli Cyclocybe aegerita
4.2.3.88 DNA and amino acid sequence determination and analysis Cyclocybe aegerita
4.2.3.88 DNA and amino acid sequence determination and analysis, functional recombinant expression in an engineered auxotrophic Escherichia coli strain Cyclocybe aegerita
4.2.3.88 expression in Escherichia coli Cyclocybe aegerita
4.2.3.135 expression in Escherichia coli Cyclocybe aegerita

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.88 (2E,6E)-farnesyl diphosphate Cyclocybe aegerita
-
 viridiflorene + diphosphate
-
 ?
4.2.3.88 (2E,6E)-farnesyl diphosphate Cyclocybe aegerita
-
 viridiflorol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.B79 Cyclocybe aegerita A0A5Q0QSI8 isoform Agr5, cf. EC 4.2.3.8
-
4.2.3.88 Cyclocybe aegerita A0A5Q0QNJ2 isoform Agr2
-
4.2.3.88 Cyclocybe aegerita A0A5Q0QNJ2 Agrocybe aegerita
-
4.2.3.88 Cyclocybe aegerita A0A5Q0QSI8 isoform Agr5
-
4.2.3.88 Cyclocybe aegerita A0A5Q0QSI8 Agrocybe aegerita
-
4.2.3.135 Cyclocybe aegerita A0A5Q0QN66 isoform Agr6
-
4.2.3.135 Cyclocybe aegerita A0A5Q0QNH9 isoform Agr7
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.B79 (2E,6E)-farnesyl diphosphate + H2O
-
 Cyclocybe aegerita viridiflorol + viridiflorene + diphosphate
-
 ?
4.2.3.88 (2E,6E)-farnesyl diphosphate
-
 Cyclocybe aegerita viridiflorene + diphosphate
-
 ?
4.2.3.88 (2E,6E)-farnesyl diphosphate
-
 Cyclocybe aegerita viridiflorol + diphosphate
-
 ?
4.2.3.88 (2E,6E)-farnesyl diphosphate
-
 Cyclocybe aegerita viridiflorene + viridiflorol + diphosphate
-
 ?
4.2.3.135 (2E,6E)-farnesyl diphosphate
-
 Cyclocybe aegerita DELTA6-protoilludene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.B79 Agr5 cf. EC 4.2.3.8 Cyclocybe aegerita
4.2.3.88 AGR2
-
 Cyclocybe aegerita
4.2.3.88 Agr5
-
 Cyclocybe aegerita
4.2.3.88 viridiflorol/viridiflorene synthase
-
 Cyclocybe aegerita
4.2.3.135 Agr6
-
 Cyclocybe aegerita
4.2.3.135 Agr7
-
 Cyclocybe aegerita

General Information

EC Number General Information Comment Organism
4.2.3.88 evolution phylogenetic analysis, detailed overview. Enzyme Agr2 belongs to the Clade II_A of cyclization in the phylogenetic tree Cyclocybe aegerita
4.2.3.88 evolution phylogenetic analysis, detailed overview. Enzyme Agr5 belongs to the Clade II_A of cyclization in the phylogenetic tree Cyclocybe aegerita
4.2.3.88 physiological function the terpene cyclase catalyzes the cyclization of farnesyl diphosphate (FPP) to viridiflorene and viridiflorol, as main products Cyclocybe aegerita