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Literature summary extracted from

  • Wang, L.; Lam, P.Y.; Lui, A.C.W.; Zhu, F.Y.; Chen, M.X.; Liu, H.; Zhang, J.; Lo, C.
    Flavonoids are indispensable for complete male fertility in rice (2020), J. Exp. Bot., 71, 4715-4728.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.162 gene CYP93G2, a single-copy gene Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.162 additional information generation of osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertion mutants, analysis of fertility and the flavonoid profiles in the flavonoid mutants, phenotypes, overview. Double mutant cyp93g1 cyp93g2 shows highly reduced male fertility compared to wild-type while the single mutants cyp93g1 and cyp93g2 do not Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.162 Fe2+ in the cytochrome P450 heme Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.162 naringenin + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 a 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.162 Oryza sativa Japonica Group Q5VRI5 cv. Zhonghua 11
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.162 anther OsF3H, CYP93G1, and CYP93G2 are entry enzymes for the production of flavonols, flavones, and flavone C-conjugates, respectively, and the corresponding genes together with OsCHS1 display high expression levels in the late developmental stages of the anthers Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.162 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group a 2-hydroxyeriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.162 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group a 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.162 CYP93G2
-
 Oryza sativa Japonica Group
1.14.14.162 OsF2H
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.162 cytochrome P450
-
 Oryza sativa Japonica Group
1.14.14.162 heme
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
1.14.14.162 malfunction detailed analysis of rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H), flavone synthase II (CYP93G1), and flavanone 2-hydroxylase (CYP93G2). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertion mutants show altered flavonoid profiles in anthers, but only the osf3h and cyp93g1 cyp93g2 mutants display reduction in seed yield Oryza sativa Japonica Group
1.14.14.162 metabolism Proposed flavonoid biosynthetic pathway in rice, overview. Flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) appears to be important, as opposed to the essential role played primarily by flavonols that has been previously reported in several plant species. Branch-point enzymes of the downstream flavonoid pathways include flavanone 3-hydroxylase (OsF3H), a flavonol pathway entry enzyme, also flavone synthase II (CYP93G1), a flavone pathway entry enzyme, aa well as flavanone 2-hydroxylase (CYP93G2), a flavone C-glycoside pathway entry enzyme Oryza sativa Japonica Group
1.14.14.162 physiological function flavanone 2-hydroxylase enzyme CYP93G2 produces 2-hydroxyflavanones from flavanones, followed by C-glycosylation and dehydration to generate flavone C-glycosides. OsF3H, CYP93G1, and CYP93G2 are entry enzymes for the production of flavonols, flavones, and flavone C-conjugates, respectively, and the corresponding genes together with OsCHS1 display high expression levels in the late developmental stages of the anthers. CYP93G2 channels the metabolite flux to flavone C-glycosides Oryza sativa Japonica Group