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Literature summary extracted from

  • Wei, J.; Zheng, Y.; Feng, H.; Qu, H.; Fan, X.; Yamaji, N.; Ma, J.F.; Xu, G.
    OsNRT2.4 encodes a dual-affinity nitrate transporter and functions in nitrate-regulated root growth and nitrate distribution in rice (2018), J. Exp. Bot., 69, 1095-1107.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.4 additional information
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nitrate-[nitrate-binding protein][side 1] in Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression shows biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. pH not specified in the publication, temperature not specified in the publication Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.4 Oryza sativa Japonica Group A2ZU80
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
7.3.2.4 root NRT2.4 is expressed mainly in the base of lateral root primordia Oryza sativa Japonica Group
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7.3.2.4 shoot NRT2.4 is expressed mainly in vascular tissues Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.4 ATP + H2O + nitrate-[nitrate-binding protein][side 1]
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Oryza sativa Japonica Group ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1]
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?

Synonyms

EC Number Synonyms Comment Organism
7.3.2.4 NRT2.4
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Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
7.3.2.4 physiological function In Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression facilitates nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. NRT2.4 does not have nitrate efflux or indole-3-acetic acid influx activity. Knockout of NRT2.4 decreases lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO3? levels. Knockout of NRT2.4 in the shoots decreases the growth and NO3--N distribution. Knockout of NRT2.4 does not affect rice growth and N uptake under conditions without N or with only NH4+ supply Oryza sativa Japonica Group