| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 7.3.2.4 | additional information | - |
nitrate-[nitrate-binding protein][side 1] | in Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression shows biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. pH not specified in the publication, temperature not specified in the publication | Oryza sativa Japonica Group |
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.3.2.4 | Oryza sativa Japonica Group | A2ZU80 | - |
- |
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.3.2.4 | root | NRT2.4 is expressed mainly in the base of lateral root primordia | Oryza sativa Japonica Group | - |
| 7.3.2.4 | shoot | NRT2.4 is expressed mainly in vascular tissues | Oryza sativa Japonica Group | - |
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.3.2.4 | ATP + H2O + nitrate-[nitrate-binding protein][side 1] | - |
Oryza sativa Japonica Group | ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1] | - |
? |
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 7.3.2.4 | NRT2.4 | - |
Oryza sativa Japonica Group |
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 7.3.2.4 | physiological function | In Xenopus oocytes, NRT2.4 alone without partner protein NAR2 coexpression facilitates nitrate uptake showing biphasic kinetics at a wide concentration range, with high- and low-affinity KM values of 0.15 and 4 mM, respectively. NRT2.4 does not have nitrate efflux or indole-3-acetic acid influx activity. Knockout of NRT2.4 decreases lateral root number and length, and the total N uptake per plant at both 0.25 and 2.5 mM NO3? levels. Knockout of NRT2.4 in the shoots decreases the growth and NO3--N distribution. Knockout of NRT2.4 does not affect rice growth and N uptake under conditions without N or with only NH4+ supply | Oryza sativa Japonica Group |