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Literature summary extracted from

  • Zang, Y.; Zha, J.; Wu, X.; Zheng, Z.; Ouyang, J.; Koffas, M.A.G.
    In vitro naringenin biosynthesis from p-coumaric acid using recombinant enzymes (2019), J. Agric. Food Chem., 67, 13430-13436.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Oryza sativa Japonica Group
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Petroselinum crispum
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Plagiochasma appendiculatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 Mg2+ 2.5 mM used in assay conditions Oryza sativa Japonica Group
6.2.1.12 Mg2+ 2.5 mM used in assay conditions Petroselinum crispum
6.2.1.12 Mg2+ 2.5 mM used in assay conditions Plagiochasma appendiculatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Petroselinum crispum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA Oryza sativa Japonica Group
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA Plagiochasma appendiculatum
-
 AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Oryza sativa Japonica Group Q6ETN3
-
-
6.2.1.12 Petroselinum crispum P14912
-
-
6.2.1.12 Plagiochasma appendiculatum A0A0D4C3L0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 His Trap column chromatography Oryza sativa Japonica Group
6.2.1.12 His Trap column chromatography Petroselinum crispum
6.2.1.12 His Trap column chromatography Plagiochasma appendiculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Petroselinum crispum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Oryza sativa Japonica Group AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumaryl-CoA ligase
-
 Petroselinum crispum
6.2.1.12 4-coumaryl-CoA ligase
-
 Oryza sativa Japonica Group
6.2.1.12 4-coumaryl-CoA ligase
-
 Plagiochasma appendiculatum
6.2.1.12 4CL1 isoform Petroselinum crispum
6.2.1.12 4CL1 isoform Plagiochasma appendiculatum
6.2.1.12 4CL3 isoform Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Petroselinum crispum
6.2.1.12 ATP
-
 Oryza sativa Japonica Group
6.2.1.12 ATP
-
 Plagiochasma appendiculatum