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Literature summary extracted from
- DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically (2018), Genes Genet. Syst., 93, 135-142.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.18.5 | gene DSH1, a conserved gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR isozyme expression analysis | Oryza sativa Japonica Group |
| 1.14.18.5 | gene DSH2, a conserved gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR isozyme expression analysis | Oryza sativa Japonica Group |
| 1.14.18.5 | gene DSH3, a monocot-specific gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR isozyme expression analysis, no transcript of DSH3 is detected | Oryza sativa Japonica Group |
| 1.14.18.5 | gene DSH4, a conserved gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR isozyme expression analysis | Oryza sativa Japonica Group |
| 1.14.18.5 | gene DSH5, a monocot-specific gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative real-time RT-PCR isozyme expression analysis, recombinant expression in Oryza sativa surD2 deletion mutant plants driven by the CaMV 35S promoter | Oryza sativa Japonica Group |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.18.5 | additional information | recombinant expression of Oryza sativa gene DSH1 in Saccharomyces cerevisiae sur2D mutant does not complement the mutant and does not lead to phytosphinganine production | Oryza sativa Japonica Group |
| 1.14.18.5 | additional information | recombinant expression of Oryza sative gene DSH5 in Saccharomyces cerevisiae sur2D mutant does not complement the mutant and does not lead to phytosphinganine production. Ectopic expression of DSH5 leads to a lethal phenotype. DSH5-overexpressing transformants show a dwarf phenotype and their mature leaves are small. Growth of the plants is severely reduced. Most of them stop gaining height, and die during the vegetative stage. Plant height of the DSH5 transformants is severely reduced | Oryza sativa Japonica Group |
| 1.14.18.5 | additional information | recombinant expression of Oryza sativa gene DSH4 in Saccharomyces cerevisiae sur2D mutant complements the mutant and leads to phytosphinganine production | Oryza sativa Japonica Group |
| 1.14.18.5 | additional information | recombinant expression of Oryza sativa gene DSH2 in Saccharomyces cerevisiae sur2D mutant complements the mutant and leads to phytosphinganine production | Oryza sativa Japonica Group |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.5 | Fe2+ | required | Oryza sativa Japonica Group |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.5 | dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | Oryza sativa Japonica Group | - |
(4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.18.5 | Oryza sativa Japonica Group | - |
- |
- |
| 1.14.18.5 | Oryza sativa Japonica Group | A0A0P0WU71 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.18.5 | leaf | highest expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | leaf | mainly | Oryza sativa Japonica Group | - |
| 1.14.18.5 | additional information | expression patterns of DSH isozymes in rice, overview | Oryza sativa Japonica Group | - |
| 1.14.18.5 | additional information | expression patterns of DSH isozymes in rice, and histochemic analysis of expression of DSH5 on the cellular level, overview | Oryza sativa Japonica Group | - |
| 1.14.18.5 | additional information | expression patterns of DSH isozymes in rice, no transcript of DSH3 is detected, overview | Oryza sativa Japonica Group | - |
| 1.14.18.5 | panicle | low expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | panicle | very low expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | root | moderate expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | root | low expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | root | higher expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | seedling | moderate to higher expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | seedling | low expression level | Oryza sativa Japonica Group | - |
| 1.14.18.5 | seedling | moderate to low expression level | Oryza sativa Japonica Group | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.18.5 | dihydroceramide + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Oryza sativa Japonica Group | (4R)-4-hydroxysphinganine ceramide + 2 ferricytochrome b5 + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.18.5 | dihydrosphingosine C4 hydroxylase | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH1 | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH2 | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH3 | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH4 | - |
Oryza sativa Japonica Group |
| 1.14.18.5 | DSH5 | - |
Oryza sativa Japonica Group |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.18.5 | cytochrome b5 | - |
Oryza sativa Japonica Group |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.18.5 | evolution | Oryza sativa has two types of DSH homologues: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species while homologues of DSH3 and DSH5 are found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs | Oryza sativa Japonica Group |
| 1.14.18.5 | malfunction | DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically | Oryza sativa Japonica Group |
| 1.14.18.5 | physiological function | dC4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively | Oryza sativa Japonica Group |
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