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Literature summary extracted from

  • Yang, Z.; Zhang, Y.; Sun, L.; Zhang, P.; Liu, L.; Yu, P.; Xuan, D.; Xiang, X.; Wu, W.; Cao, L.; Cheng, S.
    Identification of cyp703a3-3 and analysis of regulatory role of CYP703A3 in rice anther cuticle and pollen exine development (2018), Gene, 649, 63-73.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.130 gene CYP703A3, DNA and amino acid sequence determination and analysis, genetic mapping of wild-type and mutant plants, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, The cyp703a3-3 as the pollen acceptor is crossed with the wild-type Zh8015 and a japonica cultivar 02428 to generate F1 and F2 populations for genetic analysis and fine mapping. Zh8015 is crossed with 02428 to investigate the hybrid progeny fertility as the control, Agrobacterium tumefaciens-mediated transformation Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.130 additional information a cyp703a3-3 mutant is found from the progenies of indica cultivar Zhonghui8015 (Zh8015) after treating with 60Cogamma-ray radiation. The cyp703a3-3 as the pollen acceptor is crossed with the wild-type Zh8015 and a japonica cultivar 02428 to generate F1 and F2 populations for genetic analysis and fine mapping. Zh8015 is crossed with 02428 to investigate the hybrid progeny fertility as the control. Mutant cyp703a3-3 phenotype analysis, detailed overview. Expression of CYP703A3 is dramatically reduced in cyp703a3-3 mutant Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.130 Fe2+ in the cofactor Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.130 dodecanoate + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
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 7-hydroxydodecanoate + [oxidized NADPH-hemoprotein reductase] + H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.130 Oryza sativa Japonica Group Q7EZR4
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.130 anther CYP703A3 mainly expresses in anthers. CYP703A3 transcript is detectable in anthers at the anther length of 0.4-0.5 cm, when the microsporocytes are undergoing meiosis. Its strongest expression occurs at the anther length of 0.6-0.7 cm at the early stage of microspores Oryza sativa Japonica Group
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1.14.14.130 microsporocyte
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 Oryza sativa Japonica Group
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1.14.14.130 additional information CYP703A3 mRNA accumulates only in anther including different length (0.3-1.0 cm), rather than other tissues such as root, shoot, leaf and sheath Oryza sativa Japonica Group
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1.14.14.130 pollen
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 Oryza sativa Japonica Group
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1.14.14.130 seedling
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 Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.130 dodecanoate + [reduced NADPH-hemoprotein reductase] + O2
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 Oryza sativa Japonica Group 7-hydroxydodecanoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.130 CYP703A2
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 Oryza sativa Japonica Group
1.14.14.130 CYP703A3
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 Oryza sativa Japonica Group
1.14.14.130 cytochrome P450 703A2
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 Oryza sativa Japonica Group
1.14.14.130 Os08g03682 locus name Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.130 cytochrome P450
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 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
1.14.14.130 malfunction sequence analysis detects three bases (GAA) deletion in the first exon of LOC_Os08g03682, annotated as CYP703A3 with homologous sequences related to male sterility in Arabidopsis thaliana, causing the Asparagine deletion in the mutant site. Transformation of the genomic fragment of CYP703A3 into mutant cyp703a3-3 leads to recovery of the male-sterility phenotype. Both the wild-type and cyp703a3-3 mutant three-dimensional structures of CYP703A3 protein are modeled. Mutant cyp703a3-3 displays aborted microspores with exine defects and whitish anther, leading to complete male sterility. Genes involved in sporopollenin precursors formation and transportation, such as GAMYB, TDR, CYP704B2, DPW2, OsABCG26 and OsABCG15, are significantly reduced in cyp703a3-3 Oryza sativa Japonica Group
1.14.14.130 physiological function the biopolymer sporopollenin of the exine constituents plays a pivotal role in the adaptation of plants to land, such as protecting against UV light, microbial attacks, and dehydration to spores and pollen. The cytochrome P450 CYP703 family has been supposed to be a key player in this process, and CYP703A2 is a documented example of a P450 specifically involved in pollen development. Arabidopsis thaliana CYP703A2 is expressed in the anthers of developing flowers and catalyzed in-chain hydroxylation of C10 to C14 fatty acids, with a preference for lauric acid. Unlike the wide range of substrates of Arabidopsis thaliana CYP703A2, the putative orthologue of CYP703A2 in rice, CYP703A3, functions only for a specific substrate, the preferred lauric acid (dodecanoate). CYP703A3 plays vital role in anther cuticle and pollen exine development in Oryza sativa. Pollen development is a critical step in plant reproduction development Oryza sativa Japonica Group