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Literature summary extracted from

  • Lee, Y.J.; Kim, J.K.; Baek, S.A.; Yu, J.S.; You, M.K.; Ha, S.H.
    Differential regulation of an OsIspH1, the functional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, for photosynthetic pigment biosynthesis in rice leaves and seeds (2022), Front. Plant Sci., 13, 861036.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.7.4 gene ispH1, sequence comparisons and phylogenetic analysis and tree, genetic structures of isozyme genes, isozyme expression analysis, recombinant expression of wild-type and mutant isozyme OsIspH1 in Escherichia coli strain DLYT1 and in Oryza sativa mutant osisph1 plants via Agrobacterium tumefaciens strain LBA4404 transformation method Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.17.7.4 H145P site-directed mutagenesis, the mutant retains activity, complementation of two hdr mutants, including Escherichia coli DLYT1 strains and osisph1 rice plants Oryza sativa Japonica Group
1.17.7.4 H145P/K407R site-directed mutagenesis, inactive mutant, no complementation of two hdr mutants, including Escherichia coli DLYT1 strains and osisph1 rice plants Oryza sativa Japonica Group
1.17.7.4 K407R site-directed mutagenesis, the mutant retains activity, complementation of two hdr mutants, including Escherichia coli DLYT1 strains and osisph1 rice plants Oryza sativa Japonica Group
1.17.7.4 additional information a T-DNA inserted knockout mutant, osisph1, shows an albino phenotype, indicating that OsIspH1 is the only functional gene, genotyping and mutant phenotypes in the different tissues, overview Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.17.7.4 chloroplast OsIspH1 localizes into plastids Oryza sativa Japonica Group 9507
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Oryza sativa Japonica Group
-
 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ Oryza sativa Japonica Group
-
 prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.7.4 Oryza sativa Japonica Group Q6AVG6 cv. Dongjin and Ilmi
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.17.7.4 leaf
-
 Oryza sativa Japonica Group
-
1.17.7.4 additional information spatial transcript levels indicated that isozyme OsIspH1 is highly expressed in all tissues at different developmental stages, whereas isozyme OsIspH2 is barely expressed due to an early stop in exon 1 caused by splicing error, expression patterns Oryza sativa Japonica Group
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1.17.7.4 seed
-
 Oryza sativa Japonica Group
-
1.17.7.4 seedling
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Oryza sativa Japonica Group 3-methylbut-3-en-1-yl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.17.7.4 (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+
-
 Oryza sativa Japonica Group prenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.7.4 4-hydroxy-3-methylbut-2-enyl diphosphate reductase
-
 Oryza sativa Japonica Group
1.17.7.4 HDR
-
 Oryza sativa Japonica Group
1.17.7.4 isoprenoid synthesis H
-
 Oryza sativa Japonica Group
1.17.7.4 ispH
-
 Oryza sativa Japonica Group
1.17.7.4 OsIspH1
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.7.4 Ferredoxin
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
1.17.7.4 evolution phylogenetic analysis, evolutionary relationship of the rice IspH family with predicted amino acid sequences of 79 IspHs Oryza sativa Japonica Group
1.17.7.4 malfunction a T-DNA inserted knockout mutant, osisph1, shows an albino phenotype, indicating that OsIspH1 is the only functional gene. The albinism phenotype of osisph1 might also be mediated by the defective chloroplast biogenesis. When overexpressed in rice plants, the H145P/K407R double-mutated gene, OsIspH1MUT, reduces chlorophyll and carotenoid biosynthesis in the leaves and seeds Oryza sativa Japonica Group
1.17.7.4 metabolism differential regulation of an OsIspH1, the functional 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, for photosynthetic pigment biosynthesis in rice leaves and seeds. Crucial role of OsIspH1 in plastidic terpenoid biosynthesis with organ-specific differential regulation of OsIspH1 in rice plants Oryza sativa Japonica Group
1.17.7.4 additional information Oryza sativa IspH1 three-dimensional modeling based on the structure of Aquifex aeolicus IspH, PDB ID 3DNF Oryza sativa Japonica Group
1.17.7.4 physiological function the methylerythritol 4-phosphate (MEP) pathway is responsible for providing common precursors for the biosynthesis of diverse plastidial terpenoids, including chlorophylls, carotenoids, and phytohormones, in plants. In Oryza sativa, the last-step genes encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (HDR/isoprenoid synthesis H (IspH)) have been annotated in two genes (OsIspH1 and OsIspH2) in the rice genome. Crucial role of OsIspH1 in plastidic terpenoid biosynthesis with organ-specific differential regulation of OsIspH1 in rice plants Oryza sativa Japonica Group