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Literature summary extracted from

  • Zhang, Y.; Wang, X.; Luo, Y.; Zhang, L.; Yao, Y.; Han, L.; Chen, Z.; Wang, L.; Li, Y.
    OsABA8ox2, an ABA catabolic gene, suppresses root elongation of rice seedlings and contributes to drought response (2020), Crop J., 8, 480-491.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.137 gene OsABA8ox1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis Oryza sativa Japonica Group
1.14.14.137 gene OsABA8ox2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant enzyme overexpression in rice from modified pCAMBIA1303 vector using Ubi-1 promoter and Agrobacterium tumefaciens transfection method, recombinant expression of GFP-tagged enzyme in rice under control of CaMV35S promoter Oryza sativa Japonica Group
1.14.14.137 gene OsABA8ox3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression of GFP-tagged enzyme in rice under control of CaMV35S promoter Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.137 additional information OsABA8ox3 knockout is mediated by the CRISPR-Cas9 system leading to increased drought-induced abscisic acid (ABA) and indole-3-acetic acid accumulation in roots, confering increased ABA sensitivity, and promotes a more vertically oriented root system architecture (RSA) beneficial to drought tolerance. Transgenic rice lines overexpressing OsABA8ox1 show decreased levels of ABA and increased seedling vigor during cold stress Oryza sativa Japonica Group
1.14.14.137 additional information OsABA8ox3 knockout is mediated by the CRISPR-Cas9 system leading to increased drought-induced abscisic acid (ABA) and indole-3-acetic acid accumulation in roots, confering increased ABA sensitivity, and promotes a more vertically oriented root system architecture (RSA) beneficial to drought tolerance. OsABA8ox3 overexpression suppresses root elongation and increases stomatal conductance and transpiration rate. Root morphology of wild-type, OsABA8ox3 KO1 and OE2 transgenic seedlings, phenotypes, overview Oryza sativa Japonica Group
1.14.14.137 additional information OsABA8ox2 overexpression suppresses root elongation of rice seedlings, while OsABA8ox2 KO seedlings develop more vertically oriented RSA beneficial to drought tolerance. OsABA8ox2 overexpression suppresses root elongation and promotes shoot elongation, resulting in a length ratio of root to shoot of KO1 more than twice that of OE2. Soil drought stress is imposed on one-month-old seedlings by withholding irrigation for 2 weeks. Total root length, diameter of adventitious roots, and total root weight ofoverexpressing OsABA8ox2 OE2 seedlings are all much lower than those of wild-type seedlings whether under control or drought treatment. OsABA8ox2 overexpression increases stomatal conductance and transpiration rate. OsABA8ox2 knockout retards post-germination growth and increases ABA sensitivity. Phenotypes, overview Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.137 endoplasmic reticulum membrane
-
 Oryza sativa Japonica Group 5789
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.14.137 Fe2+ in the cytochrome P450 heme Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.137 (+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa Japonica Group
-
 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.137 Oryza sativa Japonica Group Q05JG2 cv. Kitaake
-
1.14.14.137 Oryza sativa Japonica Group Q0J185 cv. Kitaake
-
1.14.14.137 Oryza sativa Japonica Group Q6ZDE3 cv. Kitaake
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.137 leaf high expression level Oryza sativa Japonica Group
-
1.14.14.137 root
-
 Oryza sativa Japonica Group
-
1.14.14.137 root OsABA8ox2 is expressed mainly in roots at seedling stage and is strongly expressed in the meristematic zone of the radicle in in young adventitious roots and lateral roots, not old roots. GUS-stained roots reveal that OsABA8ox2 expression is strong in the primary meristem, and is also detected in epidermis cell, exodermis, and residual cortical parenchyma cells Oryza sativa Japonica Group
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1.14.14.137 seed OsABA8ox3 is induced early in seed germination and leads to a decrease in abscisic acid (ABA) level during seed germination Oryza sativa Japonica Group
-
1.14.14.137 seed OsABA8ox2 is induced early in seed germination and leads to a decrease in ABA level during seed germination Oryza sativa Japonica Group
-
1.14.14.137 seedling mainly in roots Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.137 (+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2
-
 Oryza sativa Japonica Group 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.137 ABA 8'-hydroxylase
-
 Oryza sativa Japonica Group
1.14.14.137 abscisic acid 8'-hydroxylase
-
 Oryza sativa Japonica Group
1.14.14.137 OsABA8ox1
-
 Oryza sativa Japonica Group
1.14.14.137 OsABA8ox2
-
 Oryza sativa Japonica Group
1.14.14.137 OsABA8ox3
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.137 cytochrome P450
-
 Oryza sativa Japonica Group
1.14.14.137 heme
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
1.14.14.137 Oryza sativa Japonica Group OsABA8ox1 is not inducible by abscisic acid additional information
1.14.14.137 Oryza sativa Japonica Group OsABA8ox3 is not induced by cold stress additional information
1.14.14.137 Oryza sativa Japonica Group OsABA8ox2 is not induced by cold stress additional information
1.14.14.137 Oryza sativa Japonica Group OsABA8ox1 is induced by cold stress within 24 h up
1.14.14.137 Oryza sativa Japonica Group OsABA8ox3 is inducible by abscisic acid. OsABA8ox3 is rapidly induced by rehydration after PEG-mimic dehydration up
1.14.14.137 Oryza sativa Japonica Group OsABA8ox2 expression in roots is markedly decreased after 0.5 h polyethylene glycol (PEG) treatment and increased after 0.5 h rehydration, implying that OsABA8ox2 is a drought-responsive gene. Soil drought stress is imposed on one-month-old seedlings by withholding irrigation for 2 weeks. Total root length, diameter of adventitious roots, and total root weight ofoverexpressing OsABA8ox2 OE2 seedlings are all much lower than those of wild-type seedlings whether under control or drought treatment. OsABA8ox2 is inducible by abscisic acid up

General Information

EC Number General Information Comment Organism
1.14.14.137 evolution the OsABA8ox family consists of three members, OsABA8ox1, OsABA8ox2 and OsABA8ox3 Oryza sativa Japonica Group
1.14.14.137 malfunction transgenic rice lines overexpressing OsABA8ox1 show decreased levels of ABA and increased seedling vigor during cold stress Oryza sativa Japonica Group
1.14.14.137 malfunction compared with wild-type seedlings, OsABA8ox3 RNAi lines show significantly increased drought stress tolerance, whereas overexpression lines are hypersensitive to drought stress Oryza sativa Japonica Group
1.14.14.137 malfunction OsABA8ox2 knockout increases drought-induced abscisic acid (ABA) and indole-3-acetic acid accumulation in roots, conferrs increased ABA sensitivity, and promotes a more vertically oriented root system architecture (RSA) beneficial to drought tolerance. OsABA8ox2 overexpression suppresses root elongation and increases stomatal conductance and transpiration rate. OsABA8ox2 knockout dramatically improves rice drought tolerance, whereas OsABA8ox2 overexpression seedlings are hypersensitive to drought stress, suggesting that OsABA8ox2 contributes to drought response in rice. Compared with wild-type, functional leaves of OsABA8ox2 knockout seedlings show higher ABA levels, whereas overexpression lines show lower ABA levels, suggesting that OsABA8ox2, as an ABA catabolic gene, modulates ABA concentration through ABA catabolism. OsABA8ox2 knockout dramatically improves rice drought tolerance Oryza sativa Japonica Group
1.14.14.137 physiological function in rice, OsABA8ox encodes abscisic acid (ABA) 8'-hydroxylase, which catalyzes the committed step of ABA catabolism. Role of ABA catabolism in root growth and development and drought response. Abscisic acid (ABA) regulates many key processes during plant growth and development, including seed maturation and dormancy, germination, leaf morphology and cold-induced pollen sterility. ABA also plays vital roles in plant responses to various environmental stresses including drought, salt, and cold. The endoplasmic reticulum localization of OsABA8ox1, OsABA8ox2 and OsABA8ox3 implies that ABA catabolism via 8'-hydroxylation occurs in the endoplasmic reticulum Oryza sativa Japonica Group
1.14.14.137 physiological function in rice, OsABA8ox encodes abscisic acid (ABA) 8'-hydroxylase, which catalyzes the committed step of ABA catabolism. Role of ABA catabolism in root growth and development and drought response. Abscisic acid (ABA) regulates many key processes during plant growth and development, including seed maturation and dormancy, germination, leaf morphology and cold-induced pollen sterility. ABA also plays vital roles in plant responses to various environmental stresses including drought, salt, and cold. OsABA8ox3 is the most highly expressed gene of the OsABA8ox family in rice leaves. The endoplasmic reticulum localization of OsABA8ox1, OsABA8ox2 and OsABA8ox3 implies that ABA catabolism via 8'-hydroxylation occurs in the endoplasmic reticulum Oryza sativa Japonica Group
1.14.14.137 physiological function in rice, OsABA8ox encodes abscisic acid (ABA) 8'-hydroxylase, which catalyzes the committed step of ABA catabolism. Role of ABA catabolism in root growth and development and drought response. OsABA8ox2, as an ABA catabolic gene, modulates ABA concentration through ABA catabolism. OsABA8ox2 suppresses root elongation of rice seedlings, increases water transpiration, and contributes to drought response through ABA catabolism. Abscisic acid (ABA) regulates many key processes during plant growth and development, including seed maturation and dormancy, germination, leaf morphology and cold-induced pollen sterility. ABA also plays vital roles in plant responses to various environmental stresses including drought, salt, and cold. The endoplasmic reticulum localization of OsABA8ox1, OsABA8ox2 and OsABA8ox3 implies that ABA catabolism via 8'-hydroxylation occurs in the endoplasmic reticulum Oryza sativa Japonica Group