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Literature summary extracted from
- Obtusifoliol 14alpha-demethylase OsCYP51G1 is involved in phytosterol synthesis and affects pollen and seed development (2020), Biochem. Biophys. Res. Commun., 529, 91-96.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.154 | gene OJ1092_A07.112, recombinant expression in transgenic Oryza sativa plants, quantitative RT-PCR enzyme expression analysis. OsCYP51G1 localization in rice cells is analyzed, OsCYP51-G1::EGFP and endoplasmic reticulum (ER)-rk::mCherry vectors are cotransformed into rice protoplasts, and transformed protoplasts are incubated in the dark at 28°C for 16-24 h and visualized using a confocal laser scanning microscope | Oryza sativa Japonica Group |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | additional information | enzyme overexpression in Oryza sativa ZH11 cell line and OsCYP51G1 RNAi knockout in Oryza sativa plants using a OsCYP51G1-CRISPR (G1-CRI) construct, the CRISPR/Cas9 vector (pYLCRISPR/Cas9Pubi-H), and an Agrobacterium faciens strain EHA105 transfection method, phenotypes, overview. Overexpressed enzyme OsCYP51G1 affects membrane integrity and phytosterol biosynthesis in rice, and OsCYP51G1 affects anther and pollen development | Oryza sativa Japonica Group |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.154 | endoplasmic reticulum membrane | - |
Oryza sativa Japonica Group | 5789 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.154 | Fe2+ | in the cytochrome P450 heme | Oryza sativa Japonica Group |  |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.154 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 | Oryza sativa Japonica Group | obtusifoliol | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.154 | Oryza sativa Japonica Group | Q84LF7 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.154 | carpel | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | internode | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | leaf lamina | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | leaf sheath | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | additional information | OsCYP51G1 is strongly expressed in most of rice organs | Oryza sativa Japonica Group | - |
| 1.14.14.154 | node | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | root | young | Oryza sativa Japonica Group | - |
| 1.14.14.154 | seedling | - |
Oryza sativa Japonica Group | - |
| 1.14.14.154 | stamen | - |
Oryza sativa Japonica Group | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.154 | 4alpha,14alpha-dimethyl-5alpha-ergosta-8,24(28)-dien-3beta-ol + [reduced NADPH-hemoprotein reductase] + O2 | obtusifoliol | Oryza sativa Japonica Group | 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | CYP51G1 | - |
Oryza sativa Japonica Group |
| 1.14.14.154 | obtusifoliol 14alpha-demethylase | - |
Oryza sativa Japonica Group |
| 1.14.14.154 | OsCYP51G1 | - |
Oryza sativa Japonica Group |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.154 | cytochrome P450 | - |
Oryza sativa Japonica Group | |
| 1.14.14.154 | heme | - |
Oryza sativa Japonica Group | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.154 | evolution | rice OsCYP51G1 shares high homology with obtusifoliol 14alpha-demethylase. OsCYP51G1 shares 54% sequence identity with OsCYP51G3, and 76%, 89%, and 90% sequence identity with, respectively, AtCYP51G1, SbCYP51G1, and ZmCYP51G1, which have been shown to catalyze the production of delta8,14-sterol from 14alpha-obtusifoliol | Oryza sativa Japonica Group |
| 1.14.14.154 | malfunction | knockdown and knockout of OsCYP51G1 results in delayed flowering, impaired membrane integrity, abnormal pollen, and reduced grain yield, whereas OsCYP51G1 overexpression leads to increased grain yield. Knockdown of OsCYP51G1 also reduces the levels of endproducts (sitosterol and stigmasterol) and increases those of upstream intermediates (24-methylene-cycloartenol and cycloeucalenol) of the OsCYP51G1-mediated sterol biosynthesis step. In contrast, overexpression of OsCYP51G1 increases the sitosterol and stigmasterol content and reduces that of cycloeucalenol. But knockdown of OsCYP51G1 by RNAi does not elicit these BR deficiency-related phenotypes, such as dwarfism, erect leaves and small seeds, nor is the leaf lamina angle sensitive to brassinolide treatment | Oryza sativa Japonica Group |
| 1.14.14.154 | metabolism | squalene is converted into 2,3-oxidosqualene by squalene epoxydiases (SQEs) or squalene SQPs and then cyclized to cycloartenol or lanosterol. A bifurcation event occurs that results in two sterol pathways, one leading to phytosterol synthesis using lanosterol as a precursor, the other to sitosterol, stigmasterol, and campesterol production utilizing cycloartenol as a precursor. The lanosterol pathway is an alternative and likely minor pathway for phytosterol biosynthesis, as evidence suggests that this pathway is not necessary for membrane sterol biosynthesis. Instead, the products of this pathway may be involved in plant defense mechanisms. In contrast, the cycloartenol pathway is the most important pathway for phytosterol production | Oryza sativa Japonica Group |
| 1.14.14.154 | physiological function | obtusifoliol 14alpha-demethylase OsCYP51G1 is involved in phytosterol synthesis and affects pollen and seed development. Rice OsCYP15G1 encodes an obtusifoliol 14alpha-demethylase, which is different from its homologue, OsCYP51G3. Rice OsCYP51G3 plays an important role in regulating phytosterol and brassinosteroid (BR) biosynthesis. Analysis of the functional difference between OsCYP51G1 and OsCYP51G3 reveals that OsCYP51G1 also encodes for an obtusifoliol 14alpha-demethylase, but unlike OsCYP51G3, OsCYP51G1 affects the biosynthesis of phytosterol, but possible does not that of BRs. OsCYP51G1 affects membrane integrity and phytosterol biosynthesis in rice, and OsCYP51G1 affects anther and pollen development. OsCYP51G1 does not influence rice grain size | Oryza sativa Japonica Group |
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