Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Xiao, T.; Mi, M.; Wang, C.; Qian, M.; Chen, Y.; Zheng, L.; Zhang, H.; Hu, Z.; Shen, Z.; Xia, Y.
    A methionine-R-sulfoxide reductase, OsMSRB5, is required for rice defense against copper toxicity (2018), Environ. Exp. Bot., 153, 45-53.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.12 gene OsMSRB5, sequence comparisons and phylogenetic analysis, quantitative real-time PCR enzyme expression analysis, recombinant ectopic expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of N- or C-terminally sGFP-tagged enzyme in onion epidermal cells Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.12 additional information generation of a mutant with osmsrb5 (PFG_3D-00669.R) with T-DNA insertion in the promoter region of the OsMSRB5 gene Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.4.12 nucleus
-
 Oryza sativa Japonica Group 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.12 L-methionine (R)-S-oxide + thioredoxin Oryza sativa Japonica Group
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 peptide-L-methionine (R)-S-oxide + thioredoxin Oryza sativa Japonica Group
-
 peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.12 Oryza sativa Japonica Group Q10L32 cv. Dongjing
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.12 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, dialysis, and ultrafiltration, to homogeneity Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.4.12 leaf
-
 Oryza sativa Japonica Group
-
1.8.4.12 additional information OsMSRB5 is mainly expressed in leaves, with low transcriptional levels of OsMSRB5 observed in seeds, stems, and roots Oryza sativa Japonica Group
-
1.8.4.12 root
-
 Oryza sativa Japonica Group
-
1.8.4.12 seed
-
 Oryza sativa Japonica Group
-
1.8.4.12 seedling
-
 Oryza sativa Japonica Group
-
1.8.4.12 stem very low content Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.12 L-methionine (R)-S-oxide + thioredoxin
-
 Oryza sativa Japonica Group L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.12 additional information OsMSRB5 has the ability to reduce free methionine-R-sulfoxide (Met-R-SO) and protein-bound-like Met-SO (dabsyl-Met-SO) to Met and dabsyl-Met, respectively Oryza sativa Japonica Group ?
-
 ?
1.8.4.12 peptide-L-methionine (R)-S-oxide + thioredoxin
-
 Oryza sativa Japonica Group peptide-L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.12 methionine sulfoxide reductase
-
 Oryza sativa Japonica Group
1.8.4.12 additional information cf. EC 1.8.4.14 Oryza sativa Japonica Group
1.8.4.12 MSR
-
 Oryza sativa Japonica Group
1.8.4.12 OsMSRB5
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
1.8.4.12 Oryza sativa Japonica Group OsMSRB5 is induced by the excess copper (Cu) treatment up

General Information

EC Number General Information Comment Organism
1.8.4.12 evolution phylogenetic analysis suggested that OsMSRB5 is a B-type MSR with similar structure to MSRBs of other species, and belongs to 2-Cys MSRB Oryza sativa Japonica Group
1.8.4.12 additional information the enzyme harbors two CXXC motifs (M1 and M3) including four non-redox-active cysteines, as well as XCGWP (M2) and RXCXNS (M4) conserved domains Oryza sativa Japonica Group
1.8.4.12 physiological function methionine-R-sulfoxide reductase OsMSRB5 is required for rice defense against copper toxicity Oryza sativa Japonica Group