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Literature summary extracted from

  • Aranda, C.; Municoy, M.; Guallar, V.; Kiebist, J.; Scheibner, K.; Ullrich, R.; Del Rio, J.; Hofrichter, M.; Martinez, A.; Gutierrez, A.
    Selective synthesis of 4-hydroxyisophorone and 4-ketoisophorone by fungal peroxygenases (2019), Catal. Sci. Technol., 9, 1398-1405.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.2.1 0.309
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
1.11.2.1 0.633
-
 isophorone at pH 7.0 and 30°C Mycothermus thermophilus
1.11.2.1 1.38
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita

Organism

EC Number Organism UniProt Comment Textmining
1.11.2.1 Chaetomium globosum
-
-
-
1.11.2.1 Chaetomium globosum DSM 62110
-
-
-
1.11.2.1 Coprinopsis cinerea
-
-
-
1.11.2.1 Cyclocybe aegerita B9W4V6
-
-
1.11.2.1 Cyclocybe aegerita TM-A1 B9W4V6
-
-
1.11.2.1 Marasmius rotula A0A023H437
-
-
1.11.2.1 Marasmius rotula DSM 25031 A0A023H437
-
-
1.11.2.1 Mycothermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.2.1 ion exchange chromatography and gel filtration Chaetomium globosum
1.11.2.1 ion exchange chromatography and gel filtration Coprinopsis cinerea
1.11.2.1 ion exchange chromatography and gel filtration Cyclocybe aegerita
1.11.2.1 ion exchange chromatography and gel filtration Marasmius rotula
1.11.2.1 ion exchange chromatography and gel filtration Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.2.1 isophorone + H2O2 72% conversion, reaction with 0.0001 mM enzyme and 5 mM H2O2 Cyclocybe aegerita (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 96% conversion, reaction with 0.00025 mM enzyme and 5 mM H2O2 Cyclocybe aegerita (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 72% conversion, reaction with 0.0001 mM enzyme and 5 mM H2O2 Cyclocybe aegerita TM-A1 (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 96% conversion, reaction with 0.00025 mM enzyme and 5 mM H2O2 Cyclocybe aegerita TM-A1 (S)-4-hydroxyisophorone + 7-hydroxyisophorone + 7-formylisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 84% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Mycothermus thermophilus 4-hydroxyisophorone + 4-ketoisophorone + 7-formylisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 95% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Chaetomium globosum 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Mycothermus thermophilus 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 95% conversion, reaction with 0.0001 mM enzyme and 2.5 mM H2O2 Chaetomium globosum DSM 62110 4-hydroxyisophorone + 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Chaetomium globosum 4-ketoisophorone + H2O
-
 ?
1.11.2.1 isophorone + H2O2 100% conversion, reaction with 0.0005 mM enzyme and 5 mM H2O2 Chaetomium globosum DSM 62110 4-ketoisophorone + H2O
-
 ?
1.11.2.1 additional information the enzyme fails to transform isophorone Coprinopsis cinerea ?
-
 ?
1.11.2.1 additional information the enzyme fails to transform isophorone Marasmius rotula ?
-
 ?
1.11.2.1 additional information the enzyme fails to transform isophorone Marasmius rotula DSM 25031 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.2.1 ? x * 44000, SDS-PAGE Coprinopsis cinerea
1.11.2.1 ? x * 46000, SDS-PAGE Cyclocybe aegerita
1.11.2.1 ? x * 32000, SDS-PAGE Marasmius rotula

Synonyms

EC Number Synonyms Comment Organism
1.11.2.1 UPO
-
 Coprinopsis cinerea
1.11.2.1 UPO
-
 Chaetomium globosum
1.11.2.1 UPO
-
 Mycothermus thermophilus
1.11.2.1 UPO
-
 Cyclocybe aegerita
1.11.2.1 UPO
-
 Marasmius rotula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.2.1 4.4
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
1.11.2.1 20.1
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita
1.11.2.1 42
-
 isophorone at pH 7.0 and 30°C Mycothermus thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.2.1 14.2
-
 isophorone at pH 7.0 and 30°C Chaetomium globosum
1.11.2.1 14.6
-
 isophorone at pH 7.0 and 30°C Cyclocybe aegerita
1.11.2.1 66.4
-
 isophorone at pH 7.0 and 30°C Mycothermus thermophilus