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Literature summary extracted from

  • Tang, J.; Shi, L.; Li, L.; Long, L.; Ding, S.
    Expression and characterization of a 9-cis-epoxycarotenoid dioxygenase from Serratia sp. ATCC 39006 capable of biotransforming isoeugenol and 4-vinylguaiacol to vanillin (2018), Biotechnol. Rep., 18, e00253 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.13.11.88 Trichloromethane the enzyme activity is substantially improved at presence of 10% (v/v) of trichloromethane (176.16% activity with isoeugenol and 208.47% activity with 4-vinylguaiacol) Serratia sp. ATCC 39006

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.88 expressed in Escherichia coli BL21(DE3) cells Serratia sp. ATCC 39006

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.88 1,10-phenanthroline 66.54% residual activity in the presence of 5 mM 1,10-phenanthroline Serratia sp. ATCC 39006
1.13.11.88 Cu2+ severe inhibition (3.17% residual activity) at 5 mM Cu2+ Serratia sp. ATCC 39006
1.13.11.88 EDTA 64.77% residual activity in the presence of 5 mM EDTA Serratia sp. ATCC 39006
1.13.11.88 ethyl acetate in the presence 10% (v/v) ethyl acetate, the enzyme shows 19.35% residual activity with isoeugenol and 35.06% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
1.13.11.88 Fe2+ 70.55% residual activity in the presence of 5 mM Fe2+ Serratia sp. ATCC 39006
1.13.11.88 Fe3+ 62.51% residual activity in the presence of 5 mM Fe3+ Serratia sp. ATCC 39006
1.13.11.88 additional information not significantly inhibited by SDS and 2,2-bipyridine Serratia sp. ATCC 39006
1.13.11.88 n-Butyl alcohol in the presence of 10% (v/v) n-butyl alcohol, the enzyme shows 38.19% residual activity with isoeugenol and 21.44% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
1.13.11.88 n-caprylic alcohol in the presence of 10% (v/v) n-caprylic acid, the enzyme shows 74.73% residual activity with isoeugenol as substrate Serratia sp. ATCC 39006
1.13.11.88 n-octane in the presence of 10% (v/v) n-octane, the enzyme shows 55.34% residual activity with isoeugenol and 70.45% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
1.13.11.88 Ni2+ 74.61% residual activity in the presence of 5 mM Ni2+ Serratia sp. ATCC 39006
1.13.11.88 petroleum ether in the presence of 10% (v/v) petroleum ether, the enzyme shows 46.05% residual activity with isoeugenol as substrate Serratia sp. ATCC 39006
1.13.11.88 Toluene in the presence of 10% (v/v) toluene, the enzyme shows 39.56% residual activity with isoeugenol and 29.36% residual activity with 4-vinylguaiacol as substrate Serratia sp. ATCC 39006
1.13.11.88 Triton X-100 66.54% residual activity in the presence of 5 mM Triton X-100 Serratia sp. ATCC 39006
1.13.11.88 Zn2+ 77.07% residual activity in the presence of 5 mM Zn2+ Serratia sp. ATCC 39006

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.88 6.31
-
 4-vinylguaiacol at pH 8.0 and 30°C Serratia sp. ATCC 39006
1.13.11.88 18.92
-
 isoeugenol at pH 8.0 and 30°C Serratia sp. ATCC 39006

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.88 Fe2+ stimulates the enzyme activity Serratia sp. ATCC 39006
1.13.11.88 additional information no significant changes of enzyme activity with Ca2+, Co2+, Mg2+, Li+, Mn2+ and Na+ Serratia sp. ATCC 39006

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.88 Serratia sp. ATCC 39006 A0A2I5TAF0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.88 Ni-NTA agarose column chromatography, and Superdex 200 gel filtration Serratia sp. ATCC 39006

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.88 4-vinylguaiacol + O2 32% conversion Serratia sp. ATCC 39006 vanillin + formaldehyde
-
 ?
1.13.11.88 isoeugenol + O2 92% conversion Serratia sp. ATCC 39006 vanillin + acetaldehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.88 ? x * 55000, SDS-PAGE Serratia sp. ATCC 39006
1.13.11.88 ? x * 55590, calculated from amino acid sequence Serratia sp. ATCC 39006

Synonyms

EC Number Synonyms Comment Organism
1.13.11.88 NCED
-
 Serratia sp. ATCC 39006

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.88 30
-
-
 Serratia sp. ATCC 39006

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.13.11.88 20 40 the enzyme presents over 80% of relative activity under a temperature range from 20 to 40°C, but almost totally inactivation as the temperature is above 45°C Serratia sp. ATCC 39006

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.88 20 40 at 20°C and 30°C, more than 90% of initial activity residue is detected after 150 min incubation. However, its activity is decreased when incubated at temperatures higher than 40°C, about 50% of its initial activity is detected at 40°C after 30 min of incubation and almost inactivated at 50°C Serratia sp. ATCC 39006

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.88 8
-
-
 Serratia sp. ATCC 39006

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.11.88 7 9 over 80% of relative activity is detected under a pH range from 7.0 to 9.0 Serratia sp. ATCC 39006

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.88 5 11 the enzyme shows a high stability at pH between 5.0 and 11.0, over 80% of initial activity is maintained after 2 h of incubation at universal buffers Serratia sp. ATCC 39006

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.88 Serratia sp. ATCC 39006 calculated from amino acid sequence
-
 5