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Literature summary extracted from

  • Tanaka, T.; Hosaka, M.; Hoshimaru, M.; Numa, S.
    Purification and properties of long-chain acyl-coenzyme -A synthetase from rat liver (1979), Eur. J. Biochem., 98, 165-172.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.3 fatty acids palmitoleate, oleate and linoleate are competitive inhibitors of the activation of each other Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.3 0.026 0.028 CoA
-
Rattus norvegicus
6.2.1.3 0.23 0.29 dephospho-CoA
-
Rattus norvegicus
6.2.1.3 0.41 0.43 4'-phosphopantetheine
-
Rattus norvegicus
6.2.1.3 1.1 1.2 pantetheine
-
Rattus norvegicus
6.2.1.3 2.3 2.4 ATP
-
Rattus norvegicus
6.2.1.3 12.5 13.3 dATP
-
Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.3 microsome
-
Rattus norvegicus
-
-
6.2.1.3 mitochondrion
-
Rattus norvegicus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.3 Mg2+ required Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.3 76000
-
x * 76000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.3
-
Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.3 liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.3 26.2
-
mitochondrial enzyme Rattus norvegicus
6.2.1.3 28.7
-
microsomal enzyme Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.2.1.3 -70°C, stable for at least 4 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.3 ATP + fatty acid + 4'-phosphopantetheine
-
Rattus norvegicus AMP + diphosphate + acyl-4'-phosphopantetheine
-
?
6.2.1.3 ATP + fatty acid + dephospho-CoA
-
Rattus norvegicus AMP + diphosphate + acyl-dephospho-CoA
-
?
6.2.1.3 ATP + fatty acid + pantetheine
-
Rattus norvegicus AMP + diphosphate + acyl-pantetheine
-
?
6.2.1.3 ATP + long-chain carboxylic acid + CoA substrate specificity of microsomal and mitochondrial enzyme are indistinguishable Rattus norvegicus AMP + diphosphate + long-chain acyl-CoA
-
?
6.2.1.3 ATP + palmitate + CoA
-
Rattus norvegicus AMP + diphosphate + palmitoyl-CoA
-
?
6.2.1.3 dATP + fatty acid + CoA
-
Rattus norvegicus dAMP + diphosphate + acyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.2.1.3 ? x * 76000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.2.1.3 35
-
2.5 min: 17-20% loss of activity, 5 min: 33-37% loss of activity, 15 min: 58-59% loss of activity Rattus norvegicus