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Literature summary extracted from

  • Wang, Q.; Wu, X.; Lu, X.; He, Y.; Ma, B.; Xu, Y.
    Efficient biosynthesis of vanillin from isoeugenol by recombinant isoeugenol monooxygenase from Pseudomonas nitroreducens Jin1 (2021), Appl. Biochem. Biotechnol., 193, 1116-1128 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.88 expressed in Escherichia coli BL21(DE3) cells Pseudomonas nitroreducens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.88 isoeugenol + O2 Pseudomonas nitroreducens
-
 vanillin + acetaldehyde
-
 ?
1.13.11.88 isoeugenol + O2 Pseudomonas nitroreducens Jin1
-
 vanillin + acetaldehyde
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.13.11.88 DMSO 10% (v/v) DMSO is the optimal co-solvent for reaction Pseudomonas nitroreducens

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.88 Pseudomonas nitroreducens C3VA26
-
-
1.13.11.88 Pseudomonas nitroreducens Jin1 C3VA26
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.88 isoeugenol + O2
-
 Pseudomonas nitroreducens vanillin + acetaldehyde
-
 ?
1.13.11.88 isoeugenol + O2 the conversion is significantly increased at high substrate concentration (50 and 100 mM) Pseudomonas nitroreducens vanillin + acetaldehyde
-
 ?
1.13.11.88 isoeugenol + O2
-
 Pseudomonas nitroreducens Jin1 vanillin + acetaldehyde
-
 ?
1.13.11.88 isoeugenol + O2 the conversion is significantly increased at high substrate concentration (50 and 100 mM) Pseudomonas nitroreducens Jin1 vanillin + acetaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.88 iem
-
 Pseudomonas nitroreducens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.88 15 20 conversion after 12 h increases from 67.0 to 84.1% with the temperature increase from 15 to 20°C, and it decreases beyond 20°C Pseudomonas nitroreducens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.13.11.88 9
-
 the enzyme is relatively stable at pH 9.0, with a residual activity over 90% after 12 h Pseudomonas nitroreducens