Literature summary extracted from

Ishibashi, T.; Ikeyama, S.; Amao, Y.
Activation of the catalytic function of formaldehyde dehydrogenase for formate reduction by single-electron reduced methylviologen (2018), New J. Chem., 42, 18508-18512 .

No PubMed abstract available

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.2.1.46	0.0875	-	formate	with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication	Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.2.1.46	formaldehyde + NAD+ + H2O	Pseudomonas sp.	-	formate + NADH + 2 H+	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.2.1.46	Pseudomonas sp.	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.2.1.46	formaldehyde + NAD+ + H2O	-	Pseudomonas sp.	formate + NADH + 2 H+	-	?
1.2.1.46	formate + reduced methyl viologen + 2 H+	single-electron reduced methyl viologen activates the enzyme activity for formate reduction to formaldehyde	Pseudomonas sp.	formaldehyde + oxidized methyl viologen + H2O	-	?
1.2.1.46	additional information	by using natural co-enzyme NADH, no formaldehyde production due to formate reduction with the enzyme is observed	Pseudomonas sp.	?	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
1.2.1.46	FldDH	-	Pseudomonas sp.

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2.1.46	0.43	-	formate	with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication	Pseudomonas sp.

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.2.1.46	NAD+	-	Pseudomonas sp.

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
1.2.1.46	0.49	-	formate	with reduced methyl viologen as cosubstrate, pH and temperature not specified in the publication	Pseudomonas sp.

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Release 2023.1 (January 2023)