Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Achard, M.E.; Tree, J.J.; Holden, J.A.; Simpfendorfer, K.R.; Wijburg, O.L.; Strugnell, R.A.; Schembri, M.A.; Sweet, M.J.; Jennings, M.P.; McEwan, A.G.
    The multi-copper-ion oxidase CueO of Salmonella enterica serovar typhimurium is required for systemic virulence (2010), Infect. Immun., 78, 2312-2319 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.16.3.4 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.16.3.4 0.0344
-
 [Cu(I)(MeCN4)]PF6+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.16.3.4 0.0526
-
 Fe2+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.16.3.4 periplasm
-
 Salmonella enterica subsp. enterica serovar Typhimurium
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.16.3.4 Salmonella enterica subsp. enterica serovar Typhimurium Q8ZRS2
-
-
1.16.3.4 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 Q8ZRS2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.16.3.4 4 Fe2+ + 4 H+ + O2 reaction of EC 1.16.3.1 Salmonella enterica subsp. enterica serovar Typhimurium 4 Fe3+ + 2 H2
-
 ?
1.16.3.4 4 Fe2+ + 4 H+ + O2 reaction of EC 1.16.3.1 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 4 Fe3+ + 2 H2
-
 ?
1.16.3.4 [Cu(I)(MeCN4)]PF6+ + 4 H+ + O2
-
 Salmonella enterica subsp. enterica serovar Typhimurium ? + 2 H2O
-
 ?
1.16.3.4 [Cu(I)(MeCN4)]PF6+ + 4 H+ + O2
-
 Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720 ? + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.16.3.4 CueO
-
 Salmonella enterica subsp. enterica serovar Typhimurium
1.16.3.4 CuiD
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.16.3.4 9.3
-
 [Cu(I)(MeCN4)]PF6+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.16.3.4 16.12
-
 Fe2+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium

General Information

EC Number General Information Comment Organism
1.16.3.4 physiological function Salmonella typhimurium CueO mutant is more sensitive to copper ions than wild-type. In a murine model of infection, the CueO mutant is significantly attenuated, although there is no significant difference in recovery from Peyer's patches and mesenteric lymph nodes. The intracellular survival of the CueO mutant in unprimed or gamma-interferon-primed murine macrophages is not statistically different from that of wild-type Salmonella typhimurium. The CueO mutant does not show greater sensitivity to hydrogen peroxide and its sensitivity to copper ions is not affected by siderophores Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.16.3.4 270
-
 [Cu(I)(MeCN4)]PF6+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium
1.16.3.4 306.7
-
 Fe2+ pH 5, temperature not specified in the publication Salmonella enterica subsp. enterica serovar Typhimurium