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Literature summary extracted from

  • Tjallinks, G.; Martin, C.; Fraaije, M.
    Enantioselective oxidation of secondary alcohols by the flavoprotein alcohol oxidase from Phanerochaete chrysosporium (2021), Arch. Biochem. Biophys., 704, 108888 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.3.18 synthesis enzyme cartalyzes selective oxidation of several secondary (S)-alcohols, so the (R)-alcohols are obtained from racemic mixtures in high enantiopurity Phanerodontia chrysosporium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.3.18 in silico docking studies. Several hydrophobic and aromatic residues in the substrate binding site create a cavity in which the substrates can comfortably undergo van der Waals and pi-stacking interactions. Consequently, oxidation of the secondary alcohols is restricted to one of the two enantiomers Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.18 F101S variant is less capable of converting the (S)-alcohols and does not convert any of the (R)-alcohols Phanerodontia chrysosporium
1.1.3.18 L317F mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium
1.1.3.18 T315S mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium
1.1.3.18 W560F mutant displays low or no significant activity with secondary alcohols and/or lower enantioselectivity than wild-type Phanerodontia chrysosporium

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.13 Phanerodontia chrysosporium T2M2J4
-
-
1.1.3.18 Phanerodontia chrysosporium T2M2J4 cf. EC 1.1.3.13
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.13 additional information enzyme additionally acts on secondary alcohols, reaction of EC 1.1.3.18. By selective oxidation of the (S)-alcohols, the (R)-alcohols can be obtained in high enantiopurity Phanerodontia chrysosporium ?
-
-
1.1.3.18 1-indanol + O2
-
 Phanerodontia chrysosporium 1-indanone + H2O2
-
 ?
1.1.3.18 1-phenylethanol + O2
-
 Phanerodontia chrysosporium 1-phenylthanone + H2O2
-
 ?
1.1.3.18 2-pentanol + O2
-
 Phanerodontia chrysosporium 2-pentanone + H2O2
-
 ?
1.1.3.18 3-butyn-2-ol + O2
-
 Phanerodontia chrysosporium 3-butyn-2-one + H2O2
-
 ?
1.1.3.18 additional information enzyme acts both on secondary alcohols, and on primary alcohols, reaction of EC 1.1.3.13. By selective oxidation of the (S)-alcohols, the (R)-alcohols can be obtained in high enantiopurity Phanerodontia chrysosporium ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.1.3.18 AOX1
-
 Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.18 FAD
-
 Phanerodontia chrysosporium