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Literature summary extracted from

  • Iermak, I.; Degtjarik, O.; Steffler, F.; Sieber, V.; Kuta Smatanova, I.
    Crystallization behaviour of glyceraldehyde dehydrogenase from Thermoplasma acidophilum (2015), Acta Crystallogr. F Struct. Biol. Commun., 71, 1475-1480 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.89 expressed in Escherichia coli BL21(DE3) cells Thermoplasma acidophilum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.89 wild type enzyme, sitting drop vapor diffusion method, using 50 mM MOPS, 50 mM sodium HEPES pH 7.5, 20% (w/v) ethylene glycol, 10% (w/v) PEG 8000, 20 mM glutamic acid (racemic), 20 mM glycine, 20 mM serine (racemic), 20 mM alanine (racemic), 20 mM lysine-HCl (racemic). Mutant enzyme F34M/S405N, sitting drop vapor diffusion method, using 50 mM MES, 50 mM imidazole pH 6.5, 8.3% (w/v) PEG 8000, 16.7% (w/v) ethylene glycol, 20 mM sodium formate, 20 mM sodium citrate, 20 mM sodium oxamate, 20 mM ammonium acetate, 20 mM sodium potassium tartrate Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.89 F34M/S405N the mutant shows a slight increase in NAD+ acceptance and a 9fold improvement in catalytic efficiency with NAD+ compared with the wild type enzyme Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NADP+ + H2O Thermoplasma acidophilum
-
 D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O Thermoplasma acidophilum ATCC 25905
-
 D-glycerate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.89 Thermoplasma acidophilum Q9HK01
-
-
1.2.1.89 Thermoplasma acidophilum ATCC 25905 Q9HK01
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.89 HisTrap column chromatography Thermoplasma acidophilum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.89 D-glyceraldehyde + NADP+ + H2O
-
 Thermoplasma acidophilum D-glycerate + NADPH + H+
-
 ?
1.2.1.89 D-glyceraldehyde + NADP+ + H2O
-
 Thermoplasma acidophilum ATCC 25905 D-glycerate + NADPH + H+
-
 ?
1.2.1.89 additional information NAD+ is a poor substrate Thermoplasma acidophilum ?
-
-
1.2.1.89 additional information NAD+ is a poor substrate Thermoplasma acidophilum ATCC 25905 ?
-
-

Subunits

EC Number Subunits Comment Organism
1.2.1.89 ? x * 56446, calculated from amino acid sequence Thermoplasma acidophilum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.89 ALDH
-
 Thermoplasma acidophilum
1.2.1.89 glyceraldehyde dehydrogenase
-
 Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.89 NADP+
-
 Thermoplasma acidophilum