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Literature summary extracted from

  • Jiang, M.; Liu, Y.; Liu, Y.; Tan, Y.; Huang, J.; Shu, Q.
    Mutation of inositol 1,3,4-trisphosphate 5/6-kinase6 impairs plant growth and phytic acid synthesis in rice (2019), Plants (Basel), 8, 114 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.7.1.134 agriculture OsITPK6 can be a desirable target of mutagenesis for breeding yield-competitive lpa rice Oryza sativa Japonica Group
2.7.1.159 agriculture OsITPK6 can be a desirable target of mutagenesis for breeding yield-competitive lpa rice Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.134 gene ositpk6, sequence comparisons, expression analysis Oryza sativa Japonica Group
2.7.1.159 gene ositpk6, sequence comparisons, expression analysis Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.134 additional information OsITPK6 mutants are generated by CRISPR/Cas9-mediated mutagenesis, generation of 23 hygromycin phosphotransferase (HPT)-positive T0 plants transformed with the CRISPR/Cas9 vector pH-itpk6. Genotyping of gene ITPK6, the ositpk6_1 (a 6-nt in-frame deletion) mutation results in the loss of two amino acids at positions 91 to 92. In contrast, all the other three mutations, i.e. ositpk6_2 (a 1-nt insertion), ositpk6_3 (a 5-nt deletion), and ositpk6_4 (a 2-nt deletion), generate a premature stop codon almost right after the mutation site and, hence, significantly shorten the encoded proteins. Consequently, the ositpk6_2, _3 and _4 mutant alleles are predicted to produce proteins of only 105, 103, and 104 amino acids, respectively. The two amino acids missing in the ositpk6_1 mutant are located in a highly conserved segment, suggesting the mutation of ositpk6_1 might have a potential functional consequence. Plant growth of mutants ositpk6_2, _3, and _4 is significantly impaired. Their panicles are significantly shorter (30.1%, 28.8%, and 29.1%, respectively) compared to wild-type parental cultivar Xidao 1, phenotypes, overview. Numbers and lengths of root from mutant plants are reduced under salt stress compared to wild-type Oryza sativa Japonica Group
2.7.1.134 P522L ositpk6 mutant, the line with the P522L amino acid substitution has agronomic performance (seed weight, germination, and seedling growth) similar to that of its wild-type parent Oryza sativa Japonica Group
2.7.1.159 additional information OsITPK6 mutants are generated by CRISPR/Cas9-mediated mutagenesis, generation of 23 hygromycin phosphotransferase (HPT)-positive T0 plants transformed with the CRISPR/Cas9 vector pH-itpk6. Genotyping of gene ITPK6, the ositpk6_1 (a 6-nt in-frame deletion) mutation results in the loss of two amino acids at positions 91 to 92. In contrast, all the other three mutations, i.e. ositpk6_2 (a 1-nt insertion), ositpk6_3 (a 5-nt deletion), and ositpk6_4 (a 2-nt deletion), generate a premature stop codon almost right after the mutation site and, hence, significantly shorten the encoded proteins. Consequently, the ositpk6_2, _3 and _4 mutant alleles are predicted to produce proteins of only 105, 103, and 104 amino acids, respectively. The two amino acids missing in the ositpk6_1 mutant are located in a highly conserved segment, suggesting the mutation of ositpk6_1 might have a potential functional consequence. Plant growth of mutants ositpk6_2, _3, and _4 is significantly impaired. Their panicles are significantly shorter (30.1%, 28.8%, and 29.1%, respectively) compared to wild-type parental cultivar Xidao 1, phenotypes, overview. Numbers and lengths of root from mutant plants are reduced under salt stress compared to wild-type Oryza sativa Japonica Group
2.7.1.159 P522L ositpk6 mutant, the line with the P522L amino acid substitution has agronomic performance (seed weight, germination, and seedling growth) similar to that of its wild-type parent Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.134 Mg2+ required Oryza sativa Japonica Group
2.7.1.159 Mg2+ required Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate Oryza sativa Japonica Group
-
 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Oryza sativa Japonica Group
-
 ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate Oryza sativa Japonica Group
-
 ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.134 Oryza sativa Japonica Group Q0J0B2
-
-
2.7.1.159 Oryza sativa Japonica Group Q0J0B2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.134 ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate
-
 Oryza sativa Japonica Group ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Oryza sativa Japonica Group ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ?
2.7.1.159 ATP + 1D-myo-inositol 1,3,4-trisphosphate
-
 Oryza sativa Japonica Group ADP + 1D-myo-inositol 1,3,4,6-tetrakisphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.134 inositol 1,3,4-trisphosphate 5/6-kinase
-
 Oryza sativa Japonica Group
2.7.1.134 inositol 1,3,4-trisphosphate 5/6-kinase6
-
 Oryza sativa Japonica Group
2.7.1.134 ITPK
-
 Oryza sativa Japonica Group
2.7.1.134 additional information see also EC 2.7.1.159 Oryza sativa Japonica Group
2.7.1.134 Os09g0518700 locus name Oryza sativa Japonica Group
2.7.1.134 OsITPK6
-
 Oryza sativa Japonica Group
2.7.1.159 inositol 1,3,4-trisphosphate 5/6-kinase
-
 Oryza sativa Japonica Group
2.7.1.159 inositol 1,3,4-trisphosphate 5/6-kinase6
-
 Oryza sativa Japonica Group
2.7.1.159 ITPK
-
 Oryza sativa Japonica Group
2.7.1.159 additional information see also EC 2.7.1.134 Oryza sativa Japonica Group
2.7.1.159 Os09g0518700 locus name Oryza sativa Japonica Group
2.7.1.159 OsITPK6
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.134 ATP
-
 Oryza sativa Japonica Group
2.7.1.159 ATP
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
2.7.1.134 Oryza sativa Japonica Group there is no significant difference in the number of leaves and roots with or without stress treatment between ositpk6_1 and wild-type additional information
2.7.1.159 Oryza sativa Japonica Group there is no significant difference in the number of leaves and roots with or without stress treatment between ositpk6_1 and wild-type additional information

General Information

EC Number General Information Comment Organism
2.7.1.134 evolution OsITPK6 belongs to subgroup III, with 12 exons and 11 introns, of the OsITPK gene family. ITPK6 is a unique gene in the ITPK gene family Oryza sativa Japonica Group
2.7.1.134 malfunction mutation of OsITPK6 not only significantly reduces the accumulation of IP6 in rice grains but also impairs plant growth and tolerance to abiotic stress. Nucleotide substitutions of gene OsITPK6 can significantly lower the phytic acid content in rice grains. Impact of ositpk6 mutations on plant growth and seed germination, panicle phenotype of mutant OsITPK6 and wild-type plants, overview. There is no significant difference in the number of leaves and roots with or without stress treatment between ositpk6_1 and wild-type Oryza sativa Japonica Group
2.7.1.159 evolution OsITPK6 belongs to subgroup III, with 12 exons and 11 introns, of the OsITPK gene family. ITPK6 is a unique gene in the ITPK gene family Oryza sativa Japonica Group
2.7.1.159 malfunction mutation of OsITPK6 not only significantly reduces the accumulation of IP6 in rice grains but also impairs plant growth and tolerance to abiotic stress. Nucleotide substitutions of gene OsITPK6 can significantly lower the phytic acid content in rice grains. Impact of ositpk6 mutations on plant growth and seed germination, panicle phenotype of mutant OsITPK6 and wild-type plants, overview. There is no significant difference in the number of leaves and roots with or without stress treatment between ositpk6_1 and wild-type Oryza sativa Japonica Group