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Literature summary extracted from

  • Bovdilova, A.; Alexandre, B.M.; Hoeppner, A.; Luis, I.M.; Alvarez, C.E.; Bickel, D.; Gohlke, H.; Decker, C.; Nagel-Steger, L.; Alseekh, S.; Fernie, A.R.; Drincovich, M.F.; Abreu, I.A.; Maurino, V.G.
    Posttranslational Modification of the NADP-malic enzyme involved in C4 photosynthesis modulates the enzymatic activity during the day (2019), Plant Cell, 31, 2525-2539 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.40 expressed in Escherichia coli Rosetta(DE3) cells Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.40 S419A the variant presents 72.3% of the wild type activity Zea mays
1.1.1.40 S419E the variant presents 8.7% of the wild type activity. The mutation dramatically decreases the affinity for the cofactor, as saturation is not observed even at NADP+ concentrations as high as 5 mM Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.40 0.0123
-
 NADP+ wild type enzyme, at pH 8.0 and 25°C Zea mays
1.1.1.40 0.1595
-
 NADP+ mutant enzyme S419A, at pH 8.0 and 25°C Zea mays
1.1.1.40 0.19
-
 (S)-malate wild type enzyme, at pH 8.0 and 25°C Zea mays
1.1.1.40 0.47
-
 (S)-malate mutant enzyme S419A, at pH 8.0 and 25°C Zea mays
1.1.1.40 2.68
-
 (S)-malate mutant enzyme S419A, at pH 8.0 and 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.40 chloroplast
-
 Zea mays 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.40 Mg2+ 10 mM used in assay conditions Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.40 (S)-malate + NADP+ Zea mays
-
 pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+ Zea mays
-
 (S)-malate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.40 Zea mays P16243
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.40 phosphoprotein phosphorylation of the enzyme at Ser419 drastically decreases its activity. Phosphorylation of isoform C4-NADP-ME at Ser419 during the first hours in the light is a cellular mechanism that fine tunes the enzymatic activity to coordinate the carbon concentration mechanism with the CO2 fixation rate, probably to avoid CO2 leakiness from bundle sheath cells Zea mays

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.40 Ni-NTA agarose column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.40 leaf
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.40 (S)-malate + NADP+
-
 Zea mays pyruvate + CO2 + NADPH + H+
-
 r
1.1.1.40 pyruvate + CO2 + NADPH + H+
-
 Zea mays (S)-malate + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.40 homotetramer 4 * 63400, calculated from amino acid sequence Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.40 C4-NADP-ME isoform Zea mays
1.1.1.40 NADP-malic enzyme
-
 Zea mays
1.1.1.40 NADP-ME
-
 Zea mays

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.40 2.1
-
 NADP+ mutant enzyme S419A, at pH 8.0 and 25°C Zea mays
1.1.1.40 8.3
-
 NADP+ mutant enzyme S419A, at pH 8.0 and 25°C Zea mays
1.1.1.40 12.2
-
 NADP+ wild type enzyme, at pH 8.0 and 25°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.40 8
-
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.40 NADP+
-
 Zea mays
1.1.1.40 NADPH
-
 Zea mays