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Literature summary extracted from

  • Park, H.L.; Kim, T.L.; Bhoo, S.H.; Lee, T.H.; Lee, S.W.; Cho, M.H.
    Biochemical characterization of the rice cinnamyl alcohol dehydrogenase gene family (2018), Molecules, 23, 2659 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.195 expressed in Escherichia coli Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.195 0.00266
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.00437
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.0053
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.00642
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.00819
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.00936
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.01072
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.01088
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.01144
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.01411
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.01618
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.02825
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.195 Zn2+ contains zinc Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.195 Oryza sativa Japonica Group Q0JA75 cultivar Dongjin
-
1.1.1.195 Oryza sativa Japonica Group Q6ZHS4 cultivar Dongjin
-
1.1.1.195 Oryza sativa Japonica Group Q7XWU3 cultivar Dongjin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.195 Ni-NTA agarose bead chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.195 leaf
-
 Oryza sativa Japonica Group
-
1.1.1.195 panicle
-
 Oryza sativa Japonica Group
-
1.1.1.195 root
-
 Oryza sativa Japonica Group
-
1.1.1.195 shoot
-
 Oryza sativa Japonica Group
-
1.1.1.195 stem
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.195 4-coumaryl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group 4-coumaryl alcohol + NADP+
-
 ?
1.1.1.195 cinnamyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group cinnamyl alcohol + NADP+
-
 ?
1.1.1.195 coniferyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group coniferyl alcohol + NADP+ ? ?
1.1.1.195 sinapyl aldehyde + NADPH + H+
-
 Oryza sativa Japonica Group sinapyl alcohol + NADP+ ? ?

Subunits

EC Number Subunits Comment Organism
1.1.1.195 ? x * 39600, isoform CAD7, calculated from amino acid sequence Oryza sativa Japonica Group
1.1.1.195 ? x * 38600, isoform CAD2, calculated from amino acid sequence Oryza sativa Japonica Group
1.1.1.195 ? x * 39100, isoform CAD6, calculated from amino acid sequence Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
1.1.1.195 CAD2 isoform Oryza sativa Japonica Group
1.1.1.195 CAD6 isoform Oryza sativa Japonica Group
1.1.1.195 CAD7 isoform Oryza sativa Japonica Group
1.1.1.195 cinnamyl alcohol dehydrogenase
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.195 0.069
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.073
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.076
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.128
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.154
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.188
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.284
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 0.311
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 9.05
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 13.86
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 30.13
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 41.19
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.195 NADPH
-
 Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
1.1.1.195 Oryza sativa Japonica Group the expression of isoform CAD2 is induced by biotic and abiotic stresses such as Xanthomonas oryzaepv. oryzae infection and UV-irradiation up
1.1.1.195 Oryza sativa Japonica Group the expression of isoform CAD6 is induced by biotic and abiotic stresses such as Xanthomonas oryzaepv. oryzae infection and UV-irradiation up

General Information

EC Number General Information Comment Organism
1.1.1.195 physiological function isoforms CAD2 is involved in developmental lignification in rice and also plays a role in the defense response against rice pathogens Oryza sativa Japonica Group
1.1.1.195 physiological function isoform CAD6 is involved in developmental lignification in rice and also plays a role in the defense response against rice pathogens Oryza sativa Japonica Group

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.195 6.8
-
 sinapyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 7.5
-
 4-coumaryl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 9.7
-
 coniferyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 11.5
-
 cinnamyl aldehyde isoform CAD6, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 13.7
-
 cinnamyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 21.2
-
 sinapyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 25.3
-
 coniferyl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 26.3
-
 4-coumaryl aldehyde isoform CAD7, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 1540.5
-
 4-coumaryl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 2369
-
 cinnamyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 5352.3
-
 sinapyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group
1.1.1.195 7154.8
-
 coniferyl aldehyde isoform CAD2, at pH 6.3 and 30°C Oryza sativa Japonica Group