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Literature summary extracted from

  • Oshima, T.; Fujita, S.; Imahori, K.
    [58] Glyceraldehyde-3-phosphate dehydrogenase from Thermus thermophilus (1982), Methods Enzymol., 89, 335-340 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.107 (NH4)2SO4 the enzyme activity is increased about 10fold in the presence of 0.045 M (NH4)2SO4 Thermus thermophilus
1.2.1.107 Na2SO4
-
 Thermus thermophilus
1.2.1.107 NaCI
-
 Thermus thermophilus
1.2.1.107 NH4Cl 25fold activation in the presence of 0.1 M Thermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.107 Cd2+ strong inhibitor Thermus thermophilus
1.2.1.107 Cu2+ strong inhibitor Thermus thermophilus
1.2.1.107 Hg2+ strong inhibitor Thermus thermophilus
1.2.1.107 Monoiodoacetamide
-
 Thermus thermophilus
1.2.1.107 monoiodoacetate potent inhibitor Thermus thermophilus
1.2.1.107 Sodium tetrathionate potent inhibitor Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.107 0.1
-
 NAD+ at pH 8.3 and 30°C Thermus thermophilus
1.2.1.107 0.3
-
 D-glyceraldehyde 3-phosphate at pH 8.3 and 30°C Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.107 Cs+ activates Thermus thermophilus
1.2.1.107 K+ activates Thermus thermophilus
1.2.1.107 Li+ activates Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+ Thermus thermophilus
-
 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.107 Thermus thermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.107 ammonium sulfate precipitation and DEAE-Sephadex A-50 gel filtration Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.107 0.11
-
 crude extract, at pH 8.3 and 30°C Thermus thermophilus
1.2.1.107 5.4
-
 purified enzyme, at pH 8.3 and 30°C Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Thermus thermophilus 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ir

Subunits

EC Number Subunits Comment Organism
1.2.1.107 homotetramer 4 * 33500, SDS-PAGE Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.107 G3P dehydrogenase
-
 Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.107 8.3
-
-
 Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.107 NAD+
-
 Thermus thermophilus