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Literature summary extracted from
- [54] Glyceraldehyde-3-phosphate dehydrogenase from pig liver (1982), Methods Enzymol., 89, 310-316 .
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.2.1.107 | the enzyme is dissociated into subunits by ATP and can be reversibly dissociated by urea | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.107 | additional information | not inhibited by 1,3-diphosphoglycerate | Sus scrofa | |
| 1.2.1.107 | NAD+ | product inhibition | Sus scrofa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.1.107 | 0.006 | - |
NAD+ | at pH 8.5 and 25°C | Sus scrofa | |
| 1.2.1.107 | 0.3 | - |
D-glyceraldehyde 3-phosphate | at pH 8.5 and 25°C | Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.107 | 145000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | Sus scrofa | - |
1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.1.107 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.2.1.107 | ammonium sulfate precipitation, DEAE-Sephadex.column chromatography, and SE-Sephadex gel filtration | Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.2.1.107 | liver | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.1.107 | D-glyceraldehyde 3-phosphate + arsenate + NAD+ | - |
Sus scrofa | 1-arsono-3-phospho-D-glycerate + NADH + H+ | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.2.1.107 | GAPDH | - |
Sus scrofa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.107 | 8.4 | 8.8 | - |
Sus scrofa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.1.107 | 7.4 | 9.6 | half-maximum activity at pH 7.4 and 9.6 | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.1.107 | NAD+ | - |
Sus scrofa | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.2.1.107 | Sus scrofa | isoelectric focusing | - |
8.8 |
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Release 2023.1 (January 2023)
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