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Literature summary extracted from

  • Chastain, C.J.; Baird, L.M.; Walker, M.T.; Bergman, C.C.; Novbatova, G.T.; Mamani-Quispe, C.S.; Burnell, J.N.
    Maize leaf PPDK regulatory protein isoform-2 is specific to bundle sheath chloroplasts and paradoxically lacks a Pi-dependent PPDK activation activity (2018), J. Exp. Bot., 69, 1171-1181 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.32 expressed in Escherichia coli BL21(DE3) cells Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.27 chloroplast
-
Zea mays 9507
-
2.7.11.32 chloroplast
-
Zea mays 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.32 Mg2+ 10 mM used in assay conditions Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.32 ADP + [pyruvate, phosphate dikinase] Zea mays
-
AMP + [pyruvate, phosphate dikinase] phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Zea mays A0A1D6IDV8 isoform PDRP2, cf. EC 2.7.11.32
-
2.7.4.27 Zea mays Q195N6 isoform PDRP1, cf. EC 2.7.11.32
-
2.7.11.32 Zea mays A0A1D6IDV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.32 Ni-NTA column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.27 bundle sheath isoform PDRP2 is strictly expressed in bundle sheath strand cells Zea mays
-
2.7.4.27 leaf
-
Zea mays
-
2.7.4.27 mesophyll isoform PDRP1 is strictly expressed in mesophyll cells Zea mays
-
2.7.11.32 bundle sheath cell isoform PDRP2 is strictly expressed in bundle sheath strand cells Zea mays
-
2.7.11.32 leaf
-
Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 additional information isoform PDRP2 lacks the PPDK-P dephosphorylation activity of the PDRP1 isoform except when PDRP2 in the assays is elevated 5- to 10fold Zea mays ?
-
-
2.7.4.27 [pyruvate, phosphate dikinase] phosphate + phosphate
-
Zea mays pyruvate, phosphate dikinase + diphosphate
-
?
2.7.11.32 ADP + [pyruvate, phosphate dikinase]
-
Zea mays AMP + [pyruvate, phosphate dikinase] phosphate
-
?
2.7.11.32 additional information isoform PDRP2 lacks the phosphotransferase activity of the bifunctional PDRP1 isoform except when PDRP2 in the assays is elevated 5-10fold Zea mays ?
-
-

Subunits

EC Number Subunits Comment Organism
2.7.4.27 ? x * 45000, SDS-PAGE Zea mays
2.7.11.32 ? x * 43000, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.4.27 GRMZM2G004880
-
Zea mays
2.7.4.27 GRMZM2G131286
-
Zea mays
2.7.4.27 PDRP1 EC 2.7.11.32 Zea mays
2.7.4.27 PDRP2
-
Zea mays
2.7.4.27 PDRP2 cf. EC 2.7.11.32 Zea mays
2.7.11.32 PDRP2
-
Zea mays
2.7.11.32 PPDK regulatory protein isoform-2
-
Zea mays