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Literature summary extracted from

  • Li, J.F.; Li, X.Q.; Liu, Y.; Yuan, F.J.; Zhang, T.; Wu, M.C.; Zhang, J.R.
    Directed modification of L-LcLDH1, an L-lactate dehydrogenase from Lactobacillus casei, to improve its specific activity and catalytic efficiency towards phenylpyruvic acid (2018), J. Biotechnol., 281, 193-198 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.27 I229A 30% decrease in Km value and 2.6fold increase in kcat value for phenylpyruvate Lacticaseibacillus casei
1.1.1.27 Q88R 17% decrease in Km value and 3fold increase in kcat value for phenylpyruvate Lacticaseibacillus casei
1.1.1.27 T235G slight decrease in specific activity towards phenylpyruvate Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.27 1.86
-
 phenylpyruvate mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 2.5 5 phenylpyruvate mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 3.5
-
 phenylpyruvate wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 5.75
-
 phenylpyruvate mutant I229A, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 6.82
-
 phenylpyruvate mutant Q88R, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 8.23
-
 phenylpyruvate wild-type, pH 5.5, 30°C Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.27 Lacticaseibacillus casei A0A2U9AUU1
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.27 15.5
-
 wild-type, substrate benzoylformic acid, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 17.4
-
 wild-type, substrate 4-hydroxyphenylpyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 25 40 wild-type, substrate pyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 66.7
-
 mutant T235G, substrate phenylpyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 72.3
-
 wild-type, substrate phenylpyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 168.4
-
 wild-type, substrate 2-oxobutanoate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 435.8
-
 mutant I229A, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 451.5
-
 mutant Q88R, substrate phenylpyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 512.4
-
 mutant I229A, substrate phenylpyruvate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 671.2
-
 wild-type, substrate oxaloacetic acid, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 680.3
-
 mutant T235G, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 798
-
 wild-type, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 4071
-
 mutant Q88R, substrate phenylpyruvate, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.27 2-oxobutanoate + NADH + H+
-
 Lacticaseibacillus casei 2-hydroxybutanoate + NAD+
-
 ?
1.1.1.27 4-hydroxyphenylpyruvate + NADH + H+
-
 Lacticaseibacillus casei L-4-hydroxyphenyllactate + NAD+
-
 ?
1.1.1.27 benzoylformic acid + NADH + H+
-
 Lacticaseibacillus casei ? + NAD+
-
 ?
1.1.1.27 oxaloacetate + NADH + H+
-
 Lacticaseibacillus casei ? + NAD+
-
 ?
1.1.1.27 phenylpyruvate + NADH + H+
-
 Lacticaseibacillus casei L-phenyllactate + NAD+
-
 ?
1.1.1.27 pyruvate + NADH + H+
-
 Lacticaseibacillus casei L-lactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.27 ? x * 36800, SDS-PAGE Lacticaseibacillus casei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.27 94.7
-
 phenylpyruvate wild-type, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 344.4
-
 phenylpyruvate mutant I229A, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 380.4
-
 phenylpyruvate mutant Q88R, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 1293
-
 phenylpyruvate mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 6467
-
 phenylpyruvate wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 17479
-
 phenylpyruvate mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.27 11.5
-
 phenylpyruvate wild-type, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 55.8
-
 phenylpyruvate mutant Q88R, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 59.9
-
 phenylpyruvate mutant I229A, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 507
-
 phenylpyruvate mutant I229A, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 1848
-
 phenylpyruvate wild-type, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei
1.1.1.27 9397
-
 phenylpyruvate mutant Q88R, presence of D-fructose-1,6-diphosphate, pH 5.5, 30°C Lacticaseibacillus casei