Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sah, S.; Lahry, K.; Talwar, C.; Singh, S.; Varshney, U.
    Monomeric NADH-oxidizing methylenetetrahydrofolate reductases from Mycobacterium smegmatis lack flavin coenzyme (2020), J. Bacteriol., 202, e00709 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.54 expression in Escherichia coli Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.54 0.0325
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 0.0632
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 0.108
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 0.145
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.54 39000
-
 gel filtration Mycolicibacterium smegmatis
1.5.1.54 41000
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.54 Mycolicibacterium smegmatis A0R6M0 isoform MTHFR1
-
1.5.1.54 Mycolicibacterium smegmatis A0R6S0 isoform MTHFR2
-
1.5.1.54 Mycolicibacterium smegmatis ATCC 700084 A0R6M0 isoform MTHFR1
-
1.5.1.54 Mycolicibacterium smegmatis ATCC 700084 A0R6S0 isoform MTHFR2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Mycolicibacterium smegmatis 5-methyltetrahydrofolate + NAD+
-
 ?
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Mycolicibacterium smegmatis ATCC 700084 5-methyltetrahydrofolate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.54 monomer 1 * 34800, SDS-PAGE Mycolicibacterium smegmatis
1.5.1.54 monomer 1 * 34400, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.54 MSMEG_6596
-
 Mycolicibacterium smegmatis
1.5.1.54 MSMEG_6649
-
 Mycolicibacterium smegmatis
1.5.1.54 MTHFR2
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.54 0.69
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 1.32
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 4.06
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 6.1
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.54 additional information no cofactor: NADPH Mycolicibacterium smegmatis
1.5.1.54 NADH
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
1.5.1.54 physiological function expression of MTHFR1 rescues an Escherichia coli MTHFR deletion mutant. The Mycolicibacterium smegmatis MTHFR1 mutant strain is partially auxotrophic for methionine and grows only poorly without methionine. The mutant strain is more sensitive to folate pathway inhibitors such as sulfachloropyridazine, 4-aminosalicylic acid, sulfamethoxazole, and trimethoprim Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.54 4.7
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 12.2
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 63
-
 5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.5.1.54 187
-
 NADH pH 7.2, 23°C Mycolicibacterium smegmatis