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Literature summary extracted from

  • Bertsch, J.; Oeppinger, C.; Hess, V.; Langer, J.; Mueller, V.
    Heterotrimeric NADH-oxidizing methylenetetrahydrofolate reductase from the acetogenic bacterium Acetobacterium woodii (2015), J. Bacteriol., 197, 1681-1689 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.54 additional information the NADH:methylenetetrahydrofolate reductase activity is not stimulated by ferredoxin Acetobacterium woodii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.1.54 Fe complex contains 23.5 Fe and 24.5 S, corresponding to predicted six [4Fe4S] clusters in MetV and RnfC2 Acetobacterium woodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.54 130000
-
 PAGE Acetobacterium woodii

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.54 Acetobacterium woodii H6LBX9 cf. EC 1.5.1.20
-
1.5.1.54 Acetobacterium woodii DSM 1030 H6LBX9 cf. EC 1.5.1.20
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.54
-
 Acetobacterium woodii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.54 248
-
 substrate 5,10-methylenetetrahydrofolate, pH 7.0, 30°C Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Acetobacterium woodii 5-methyltetrahydrofolate + NAD+
-
 ir
1.5.1.54 5,10-methylenetetrahydrofolate + NADH + H+
-
 Acetobacterium woodii DSM 1030 5-methyltetrahydrofolate + NAD+
-
 ir
1.5.1.54 additional information no substrate: ferredoxin Acetobacterium woodii ?
-
-
1.5.1.54 additional information no substrate: ferredoxin Acetobacterium woodii DSM 1030 ?
-
-
1.5.1.54 NADH + H+ + ferricyanide
-
 Acetobacterium woodii NAD+ + ferrocyanide
-
 r
1.5.1.54 NADH + H+ + ferricyanide
-
 Acetobacterium woodii DSM 1030 NAD+ + ferrocyanide
-
 r
1.5.1.54 NADH + H+ + methylviologen
-
 Acetobacterium woodii NAD+ + reduced methylviologen
-
 r
1.5.1.54 NADH + H+ + methylviologen
-
 Acetobacterium woodii DSM 1030 NAD+ + reduced methylviologen
-
 r

Subunits

EC Number Subunits Comment Organism
1.5.1.54 heterotrimer 1 * 71000, i.e.subunit RnfC2, 1 * 32800, i.e. subunit MetF, 1 * 22700, i.e. subunit MetV, respectively Acetobacterium woodii

Synonyms

EC Number Synonyms Comment Organism
1.5.1.54 metF
-
 Acetobacterium woodii

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.54 FMN complex contains 2 flavin mononucleotides Acetobacterium woodii
1.5.1.54 [4Fe-4S]-center complex contains 23.5 Fe and 24.5 S, corresponding to predicted six [4Fe4S] clusters in MetV and RnfC2 Acetobacterium woodii