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Literature summary extracted from

  • Tomschy, A.; Glockshuber, R.; Jaenicke, R.
    Functional expression of D-glyceraldehyde-3-phosphate dehydrogenase from the hyperthermophilic eubacterium Thermotoga maritima in Escherichia coli Authenticity and kinetic properties of the recombinant enzyme (1993), Eur. J. Biochem., 214, 43-50 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.107 expressed in Escherichia coli W3CG cells Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.107 arsenate the enzyme is allosterically inhibited at concentrations above 10 mM Thermotoga maritima
1.2.1.107 D-glyceraldehyde 3-phosphate the enzyme is allosterically inhibited at concentrations above 1 mM Thermotoga maritima
1.2.1.107 L-Glyceraldehyde 3-phosphate the enzyme is allosterically inhibited at concentrations above 1 mM Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.107 0.012
-
 NAD+ at pH 8.0 and 40°C Thermotoga maritima
1.2.1.107 3.3
-
 D-glyceraldehyde 3-phosphate at pH 8.0 and 40°C Thermotoga maritima
1.2.1.107 18
-
 arsenate at pH 8.0 and 40°C Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+ Thermotoga maritima
-
 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ?
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+ Thermotoga maritima MSB 8
-
 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.107 Thermotoga maritima
-
-
-
1.2.1.107 Thermotoga maritima MSB 8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.107 phenyl-Sepharose column chromatography and DEAE-cellulose column chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Thermotoga maritima 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ?
1.2.1.107 D-glyceraldehyde 3-phosphate + arsenate + NAD+
-
 Thermotoga maritima MSB 8 1-arsono-3-phospho-D-glycerate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.107 ? x * 40000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.2.1.107 D-glyceraldehyde-3-phosphate dehydrogenase
-
 Thermotoga maritima
1.2.1.107 GAPDH
-
 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.107 NAD+
-
 Thermotoga maritima