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Literature summary extracted from

  • Lund, S.; Courtney, T.; Williams, G.J.
    Probing the substrate promiscuity of isopentenyl phosphate kinase as a platform for hemiterpene analogue production (2019), ChemBioChem, 20, 2217-2221 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.26 expression in Escherichia coli Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.26 0.028
-
 isopentenyl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.052
-
 2-methylprop-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.14
-
 dimethylallyl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.231
-
 3-bromobut-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.322
-
 (2E)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.445
-
 pent-4-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.47
-
 but-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.543
-
 pent-4-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.67
-
 but-3-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 0.718
-
 (2Z)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.26 Thermoplasma acidophilum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.26 ATP + (2E)-hex-2-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + (2E)-hex-2-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + (2Z)-hex-2-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + (2Z)-hex-2-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + 2-methylprop-2-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + 2-methylprop-2-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + 3-bromobut-3-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + 3-bromobut-3-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + but-2-yn-1-yl phosphate
-
 Thermoplasma acidophilum ADP + but-2-yn-1-yl diphosphate
-
 ?
2.7.4.26 ATP + but-3-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + but-3-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + but-3-yn-1-yl phosphate
-
 Thermoplasma acidophilum ADP + but-3-yn-1-yl diphosphate
-
 ?
2.7.4.26 ATP + dimethylallyl phosphate
-
 Thermoplasma acidophilum ADP + dimethylallyl diphosphate
-
 ?
2.7.4.26 ATP + hex-5-yn-1-yl phosphate
-
 Thermoplasma acidophilum ADP + hex-5-yn-1-yl diphosphate
-
 ?
2.7.4.26 ATP + isopentenyl phosphate
-
 Thermoplasma acidophilum ADP + isopentenyl diphosphate
-
 ?
2.7.4.26 ATP + pent-4-en-1-yl phosphate
-
 Thermoplasma acidophilum ADP + pent-4-en-1-yl diphosphate
-
 ?
2.7.4.26 ATP + pent-4-en-2-yl phosphate
-
 Thermoplasma acidophilum ADP + pent-4-en-2-yl diphosphate
-
 ?
2.7.4.26 ATP + pent-4-yn-1-yl phosphate
-
 Thermoplasma acidophilum ADP + pent-4-yn-1-yl diphosphate
-
 ?
2.7.4.26 additional information no substrates: geranyl monophosphate, neryl monophosphate, and farnesyl monophosphate Thermoplasma acidophilum ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.4.26 Ta0103
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.26 12
-
 2-methylprop-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 18
-
 (2E)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 32
-
 dimethylallyl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 34
-
 but-3-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 44
-
 isopentenyl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 44
-
 pent-4-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 92
-
 pent-4-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 112
-
 (2Z)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 187
-
 but-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 226
-
 3-bromobut-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.4.26 50
-
 but-3-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 55
-
 (2E)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 99
-
 pent-4-yn-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 160
-
 (2Z)-hex-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 170
-
 pent-4-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 230
-
 dimethylallyl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 240
-
 2-methylprop-2-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 400
-
 but-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 980
-
 3-bromobut-3-en-1-yl phosphate pH 8.0, 30°C Thermoplasma acidophilum
2.7.4.26 1600
-
 isopentenyl phosphate pH 8.0, 30°C Thermoplasma acidophilum