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Literature summary extracted from

  • Zhang, Z.; Liang, X.; Lu, L.; Xu, Z.; Huang, J.; He, H.; Peng, X.
    Two glyoxylate reductase isoforms are functionally redundant but required under high photorespiration conditions in rice (2020), BMC Plant Biol., 20, 357 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.79 expressed in Escherichia coli Rosetta (DE3) cells Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.79 oxalate when using NADPH as cofactor, the Ki value of oxalate for isoform GR1 is 21.2 mM and that for isoform GR2 is 290.8 mM. When using NADH as cofactor, the Ki values of oxalate are much lower, 3.6mM for isoform GR1, and 8.2mM for isoform GR2 Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.79 0.0176
-
 NADPH isoform GR1, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.0304
-
 glyoxylate isoform GR1, with NADPH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.053
-
 NADPH isoform GR2, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.0721
-
 glyoxylate isoform GR2, with NADPH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.1446
-
 glyoxylate isoform GR2, with NADH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.2677
-
 glyoxylate isoform GR1, with NADH as cosubstrate, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.4039
-
 NADH isoform GR2, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 0.4202
-
 NADH isoform GR1, at pH 7.4 and 30°C Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.79 chloroplast isoform GR1 Oryza sativa Japonica Group 9507
-
1.1.1.79 cytosol isoform GR1 Oryza sativa Japonica Group 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.79 glycolate + NADP+ Oryza sativa Japonica Group
-
 glyoxylate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.79 Oryza sativa Japonica Group
-
 cultivar Zhonghua 11
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.79 His-tag affinity Ni-IDA resin column chromatography Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.79 glume
-
 Oryza sativa Japonica Group
-
1.1.1.79 leaf
-
 Oryza sativa Japonica Group
-
1.1.1.79 leaf sheath
-
 Oryza sativa Japonica Group
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.79 glycolate + NADP+
-
 Oryza sativa Japonica Group glyoxylate + NADPH + H+
-
 ?
1.1.1.79 glyoxylate + NADH + H+ NADPH-dependent activity is much higher than the NADH-dependent activity Oryza sativa Japonica Group glycolate + NAD+
-
 ?
1.1.1.79 glyoxylate + NADPH + H+ NADPH-dependent activity is much higher than the NADH-dependent activity Oryza sativa Japonica Group glycolate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.79 ? x * 30000, isoform GR1, SDS-PAGE Oryza sativa Japonica Group
1.1.1.79 ? x * 37000, isoform GR2, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
1.1.1.79 GR1 isoform Oryza sativa Japonica Group
1.1.1.79 GR2 isoform Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.79 7 7.5
-
 Oryza sativa Japonica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.79 NADPH the enzyme prefers NADPH to NADH as cofactor Oryza sativa Japonica Group

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.79 3.6
-
 oxalate isoform GR1, with glyoxylate and NADH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 8.2
-
 oxalate isoform GR2, with glyoxylate and NADH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 212
-
 oxalate isoform GR1, with glyoxylate and NADPH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group
1.1.1.79 290.8
-
 oxalate isoform GR2, with glyoxylate and NADPH as substrates, at pH 7.4 and 30°C Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
1.1.1.79 physiological function isoforms GR1 and GR2 function redundantly in detoxifying glyoxylate in rice plants under normal growth conditions, whereas both are simultaneously required under high photorespiration conditions Oryza sativa Japonica Group