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Literature summary extracted from

  • Taylor, Z.W.; Raushel, F.M.
    Manganese-induced substrate promiscuity in the reaction catalyzed by phosphoglutamine cytidylyltransferase from Campylobacter jejuni (2019), Biochemistry, 58, 2144-2151 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.103 gene Cj1416, recombinant expression of His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) Campylobacter jejuni subsp. jejuni

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.103 Mg2+ limited activity Campylobacter jejuni subsp. jejuni
2.7.7.103 Mn2+ required, can replace Mg2+, manganese-induced promiscuity of Cj1416 Campylobacter jejuni subsp. jejuni
2.7.7.103 additional information limited activity with Mg2+, expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP Campylobacter jejuni subsp. jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.103 CTP + N5-phospho-L-glutamine Campylobacter jejuni subsp. jejuni
-
 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine Campylobacter jejuni subsp. jejuni NCTC 11168
-
 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine Campylobacter jejuni subsp. jejuni ATCC 700819
-
 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.103 Campylobacter jejuni subsp. jejuni Q0P8J8 serotype O2
-
2.7.7.103 Campylobacter jejuni subsp. jejuni ATCC 700819 Q0P8J8 serotype O2
-
2.7.7.103 Campylobacter jejuni subsp. jejuni NCTC 11168 Q0P8J8 serotype O2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.103 recombinant His6-tagged enzyme from Escherichia coli strain Rosetta (DE3) by nickel affinity chromatography Campylobacter jejuni subsp. jejuni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.103 CTP + 3'-phosphocytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni diphosphate + 3'-phospho-5'-cytidine diphosphoramidate
-
 ?
2.7.7.103 CTP + 3'-phosphocytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni NCTC 11168 diphosphate + 3'-phospho-5'-cytidine diphosphoramidate
-
 ?
2.7.7.103 CTP + 3'-phosphocytidine 5'-diphosphoramidate
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 diphosphate + 3'-phospho-5'-cytidine diphosphoramidate
-
 ?
2.7.7.103 CTP + 3-phospho-D-glycerate
-
 Campylobacter jejuni subsp. jejuni diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid
-
 ?
2.7.7.103 CTP + 3-phospho-D-glycerate
-
 Campylobacter jejuni subsp. jejuni NCTC 11168 diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid
-
 ?
2.7.7.103 CTP + 3-phospho-D-glycerate
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 diphosphate + 2-hydroxy-2-oxo-1,3,2lambda5-dioxaphospholane-4-carboxylic acid
-
 ?
2.7.7.103 CTP + 3-phospho-D-glycerate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-3-D-glycerate
-
 ?
2.7.7.103 CTP + arsenate
-
 Campylobacter jejuni subsp. jejuni diphosphate + ?
-
 ?
2.7.7.103 CTP + ethanolamine phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-ethanolamine
-
 ?
2.7.7.103 CTP + ethanolamine phosphate
-
 Campylobacter jejuni subsp. jejuni NCTC 11168 diphosphate + CDP-ethanolamine
-
 ?
2.7.7.103 CTP + ethanolamine phosphate
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 diphosphate + CDP-ethanolamine
-
 ?
2.7.7.103 CTP + glycerol-1-phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + 2-hydroxy-4-(hydroxymethyl)-1,3,2lambda5-dioxaphospholan-2-one + CMP
-
 ?
2.7.7.103 CTP + glycerol-1-phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-glycerol
-
 ?
2.7.7.103 CTP + glycerol-2-phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + 2-hydroxy-4-(hydroxymethyl)-1,3,2lambda5-dioxaphospholan-2-one + CMP
-
 ?
2.7.7.103 CTP + glycerol-2-phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-2-glycerol
-
 ?
2.7.7.103 CTP + L-serine phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-L-serine
-
 ?
2.7.7.103 CTP + methyl phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + O-methyl-CDP
-
 ?
2.7.7.103 CTP + methyl phosphonate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CMP methyl phosphonate
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine
-
 Campylobacter jejuni subsp. jejuni diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine
-
 Campylobacter jejuni subsp. jejuni NCTC 11168 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + N5-phospho-L-glutamine
-
 Campylobacter jejuni subsp. jejuni ATCC 700819 diphosphate + N5-(cytidine 5'-diphosphoramidyl)-L-glutamine
-
 ?
2.7.7.103 CTP + phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP
-
 ?
2.7.7.103 CTP + phosphoramidate
-
 Campylobacter jejuni subsp. jejuni diphosphate + cytidine 5'-diphosphoramidate
-
 ?
2.7.7.103 CTP + serinol phosphate
-
 Campylobacter jejuni subsp. jejuni diphosphate + CDP-serinol
-
 ?
2.7.7.103 additional information in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis Campylobacter jejuni subsp. jejuni ?
-
-
2.7.7.103 additional information in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis Campylobacter jejuni subsp. jejuni NCTC 11168 ?
-
-
2.7.7.103 additional information in the presence of MnCTP, Cj1416 can directly catalyze the formation of cytidine diphosphoramidate from phosphoramidate and MnCTP. In addition, it catalyzes the formation of 12 different reaction products using L-glutamine phosphate, phosphoramidate, methyl phosphate, methyl phosphonate, phosphate, arsenate, ethanolamine phosphate, glycerol-1-phosphate, glycerol-2-phosphate, serinol phosphate, L-serine phosphate, or 3-phospho-D-glycerate as the nucleophile to displace pyrophosphate from CTP. No activity is detected with MgATP as a substrate. Expansion in the substrate profile of Cj1416 when the reaction is catalyzed in the presence of MnCTP, substrate specificity of Cj1416, overview. NMR product analysis Campylobacter jejuni subsp. jejuni ATCC 700819 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.7.7.103 Cj1416
-
 Campylobacter jejuni subsp. jejuni
2.7.7.103 CTP/phosphoglutamine cytididylyltransferase
-
 Campylobacter jejuni subsp. jejuni
2.7.7.103 phosphoglutamine cytidylyltransferase
-
 Campylobacter jejuni subsp. jejuni

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.103 30
-
 assay at Campylobacter jejuni subsp. jejuni

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.103 8
-
 assay at Campylobacter jejuni subsp. jejuni

General Information

EC Number General Information Comment Organism
2.7.7.103 metabolism Campylobacter jejuni, is a Gram-negative pathogen that contains a unique O-methyl phosphoramidate (MeOPN) on its capsular polysaccharide. Enzyme CTP/phosphoglutamine cytididylyltransferase (Cj1416) catalyzes the second step of the biosynthesis pathway, the displacement of diphosphate from MgCTP by L-glutamine phosphate to form CDP-L-glutamine Campylobacter jejuni subsp. jejuni
2.7.7.103 physiological function Campylobacter jejuni, is a Gram-negative pathogen that contains a unique O-methyl phosphoramidate (MeOPN) on its capsular polysaccharide. Enzyme CTP/phosphoglutamine cytididylyltransferase (Cj1416) catalyzes the second step of the biosynthesis pathway, the displacement of diphosphate from MgCTP by L-glutamine phosphate to form CDP-L-glutamine Campylobacter jejuni subsp. jejuni