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Literature summary extracted from

  • Admiraal, S.; Khosla, C.; Walsh, C.
    The loading and initial elongation modules of rifamycin synthetase collaborate to produce mixed aryl ketide products (2002), Biochemistry, 41, 5313-5324 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.74 additional information expression of the N-terminal 260 kDa loading and initial elongation modules of rifamycin synthetase Amycolatopsis mediterranei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.74 1.1
-
 benzoate pH 7.2, 30°C Amycolatopsis mediterranei
6.2.1.74 1.1
-
 3-hydroxybenzoate pH 7.2, 30°C Amycolatopsis mediterranei

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.74 Amycolatopsis mediterranei O54666
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
6.2.1.74 phospholipoprotein activity requires phosphopantetheinylation of the thiolation domain of RifA Amycolatopsis mediterranei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.74 ATP + 3-amino-5-hydroxybenzoate + a holo-[acyl-carrier protein]
-
 Amycolatopsis mediterranei 3-amino-5-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate
-
 ?
6.2.1.74 ATP + 3-hydroxybenzoate + a holo-[acyl-carrier protein]
-
 Amycolatopsis mediterranei 3-hydroxybenzoyl-[acyl-carrier protein] + AMP + diphosphate
-
 ?
6.2.1.74 ATP + benzoate + a holo-[acyl-carrier protein]
-
 Amycolatopsis mediterranei benzoyl-[acyl-carrier protein] + AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.74 rifA
-
 Amycolatopsis mediterranei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.74 0.0018
-
 benzoate pH 7.2, 30°C Amycolatopsis mediterranei
6.2.1.74 0.008
-
 3-hydroxybenzoate pH 7.2, 30°C Amycolatopsis mediterranei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.74 0.00167
-
 benzoate pH 7.2, 30°C Amycolatopsis mediterranei
6.2.1.74 0.00767
-
 3-hydroxybenzoate pH 7.2, 30°C Amycolatopsis mediterranei