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Literature summary extracted from

  • Eprintsev, A.; Gataullina, M.
    Isolation, purification, and properties of peroxisomal malate dehydrogenase from maize mesophyll (2018), Appl. Biochem. Microbiol., 54, 320-323 .
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.37 0.26
-
 oxaloacetate at pH 8.0 and 25°C Zea mays
1.1.1.37 0.33
-
 NADH at pH 8.0 and 25°C Zea mays
1.1.1.37 11.6
-
 (S)-malate at pH 8.0 and 25°C Zea mays
1.1.1.37 129
-
 NAD+ at pH 8.0 and 25°C Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 peroxisome
-
 Zea mays 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 73400
-
 gel filtration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 oxaloacetate + NADH + H+ Zea mays
-
 (S)-malate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Zea mays
-
 cultivar Voronezhskaya 76
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 DEAE-Sephacelcolumn chromatography and Sephadex G-25 gel filtration Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 leaf
-
 Zea mays
-
1.1.1.37 mesophyll
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 3700
-
 enzyme from homogenate, at pH 8.0 and 25°C Zea mays
1.1.1.37 533000
-
 after 144fold purification, at pH 8.0 and 25°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 oxaloacetate + NADH + H+
-
 Zea mays (S)-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 homodimer 2 * 35500, calculated from amino acid sequence Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 MDH
-
 Zea mays
1.1.1.37 peroxisomal malate dehydrogenase
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 9
-
 pH optimum for reverse reaction Zea mays
1.1.1.37 9.5
-
 pH optimum for forward reaction Zea mays