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Literature summary extracted from

  • Niazi, A.; Bariat, L.; Riondet, C.; Carapito, C.; Mhamdi, A.; Noctor, G.; Reichheld, J.
    Cytosolic isocitrate dehydrogenase from Arabidopsis thaliana is regulated by glutathionylation (2019), Antioxidants (Basel), 8, 16 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expressed in Escherichia coli BL21 cells Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 C363S the mutation does not perturb the enzymatic characteristics of the enzyme. However, the mutant is less affected by nitrosoglutathione treatment than the wild type enzyme Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.42 GSSG increasing GSSG concentrations progressively inhibit the enzyme activity, almost complete inhibition at 1 mM Arabidopsis thaliana
1.1.1.42 additional information increasing concentrations of H2O2 hardly affect the enzyme activity Arabidopsis thaliana
1.1.1.42 nitrosoglutathione increasing nitrosoglutathione concentrations progressively inhibit the enzyme activity, complete inhibition at 2 mM Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.028
-
NADP+ wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.42 0.095
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.42 0.099
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
Arabidopsis thaliana 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+ 5 mM used in assay conditions Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 90000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Arabidopsis thaliana
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Arabidopsis thaliana Q9SRZ6 ecotype Columbia-0
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.1.1.42 enzyme activity is affected by oxidative agents like oxidized glutathione and nitrosoglutathione through modifications of conserved Cys residues in the protein Arabidopsis thaliana

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.1.1.42 glutathionylation S-glutathionylation of Cys363 is involved in the inhibition of the cytosolic enzyme activity upon nitrosoglutathione treatments Arabidopsis thaliana
1.1.1.42 S-nitrosylation
-
Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 Ni2+-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+
-
Arabidopsis thaliana 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.42 homodimer 2 * 48300, His-tagged enzyme, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 cICDH
-
Arabidopsis thaliana
1.1.1.42 NADP-dependent isocitrate dehydrogenase
-
Arabidopsis thaliana
1.1.1.42 NADP-ICDH
-
Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.42 4.31
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.42 4.93
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.42 5.81
-
NADP+ wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 8.5
-
-
Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.42 7.5 8.5 high activity between pH 7.5 and 8.5 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.42 45.4
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
1.1.1.42 49.7
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana