EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.42 | expressed in Escherichia coli BL21 cells | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.42 | C363S | the mutation does not perturb the enzymatic characteristics of the enzyme. However, the mutant is less affected by nitrosoglutathione treatment than the wild type enzyme | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | GSSG | increasing GSSG concentrations progressively inhibit the enzyme activity, almost complete inhibition at 1 mM | Arabidopsis thaliana | |
1.1.1.42 | additional information | increasing concentrations of H2O2 hardly affect the enzyme activity | Arabidopsis thaliana | |
1.1.1.42 | nitrosoglutathione | increasing nitrosoglutathione concentrations progressively inhibit the enzyme activity, complete inhibition at 2 mM | Arabidopsis thaliana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 0.028 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
1.1.1.42 | 0.095 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
1.1.1.42 | 0.099 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.42 | cytosol | - |
Arabidopsis thaliana | 5829 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | Mg2+ | 5 mM used in assay conditions | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 90000 | - |
gel filtration | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | Arabidopsis thaliana | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.42 | Arabidopsis thaliana | Q9SRZ6 | ecotype Columbia-0 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.1.1.42 | enzyme activity is affected by oxidative agents like oxidized glutathione and nitrosoglutathione through modifications of conserved Cys residues in the protein | Arabidopsis thaliana |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
1.1.1.42 | glutathionylation | S-glutathionylation of Cys363 is involved in the inhibition of the cytosolic enzyme activity upon nitrosoglutathione treatments | Arabidopsis thaliana |
1.1.1.42 | S-nitrosylation | - |
Arabidopsis thaliana |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.42 | Ni2+-Sepharose column chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.42 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.42 | isocitrate + NADP+ | - |
Arabidopsis thaliana | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.42 | homodimer | 2 * 48300, His-tagged enzyme, calculated from amino acid sequence | Arabidopsis thaliana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.42 | cICDH | - |
Arabidopsis thaliana |
1.1.1.42 | NADP-dependent isocitrate dehydrogenase | - |
Arabidopsis thaliana |
1.1.1.42 | NADP-ICDH | - |
Arabidopsis thaliana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 4.31 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
1.1.1.42 | 4.93 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana | |
1.1.1.42 | 5.81 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 8.5 | - |
- |
Arabidopsis thaliana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.42 | 7.5 | 8.5 | high activity between pH 7.5 and 8.5 | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.42 | NADP+ | - |
Arabidopsis thaliana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.42 | 45.4 | - |
isocitrate | mutant enzyme C3636S, at pH 7.5 and 25°C | Arabidopsis thaliana | |
1.1.1.42 | 49.7 | - |
isocitrate | wild type enzyme, at pH 7.5 and 25°C | Arabidopsis thaliana |