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Literature summary extracted from

  • Luo, X.; Zhang, Y.; Yin, F.; Hu, G.; Jia, Q.; Yao, C.; Fu, Y.
    Enzymological characterization of a novel D-lactate dehydrogenase from Lactobacillus rossiae and its application in D-phenyllactic acid synthesis (2020), 3 Biotech, 10, 101 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.28 synthesis recombinant Escherichia coli expressing D-lactate dehydrogenase, without coexpression of a cofactor regeneration system, can produce 20.5 g/l D-phenyllactic acid with enantiomeric excess above 99% from phenylpyruvic acid in a fed-batch biotransformation process, with a productivity of 49.2 g/l per day Furfurilactobacillus rossiae

General Stability

EC Number General Stability Organism
1.1.1.28 stable in a neutral to alkaline environment below 35 °C Furfurilactobacillus rossiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 Al3+ 1 mM, 13% residual activity Furfurilactobacillus rossiae
1.1.1.28 Ba2+ 1 mM, 88% residual activity Furfurilactobacillus rossiae
1.1.1.28 Cu2+ 1 mM, 83% residual activity Furfurilactobacillus rossiae
1.1.1.28 Fe2+ 1 mM, 66% residual activity Furfurilactobacillus rossiae
1.1.1.28 Fe3+ 1 mM, 73% residual activity Furfurilactobacillus rossiae
1.1.1.28 Mg2+ 1 mM, 85% residual activity Furfurilactobacillus rossiae
1.1.1.28 Mn2+ 1 mM, 97% residual activity Furfurilactobacillus rossiae
1.1.1.28 additional information not inhibitory: EDTA at 2%, Ca2+ Furfurilactobacillus rossiae
1.1.1.28 Ni2+ 1 mM, 80% residual activity Furfurilactobacillus rossiae
1.1.1.28 SDS 1%, 9% residual activity Furfurilactobacillus rossiae
1.1.1.28 Triton X-100 1%, 81% residual activity Furfurilactobacillus rossiae
1.1.1.28 Tween 80 1%, 89% residual activity Furfurilactobacillus rossiae
1.1.1.28 Zn2+ 1 mM, 114% residual activity Furfurilactobacillus rossiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 2.83
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.28 Al3+ 1 mM, 109% of initial activity Furfurilactobacillus rossiae
1.1.1.28 Co2+ 1 mM, 115% of initial activity Furfurilactobacillus rossiae

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.28 butyl acetate 2%, 127% of initial activity Furfurilactobacillus rossiae
1.1.1.28 cyclohexane 2%, 146% of initial activity Furfurilactobacillus rossiae
1.1.1.28 dibutyl phthalate 2%, 90% of initial activity Furfurilactobacillus rossiae
1.1.1.28 dichloromethane 2%, 84% of initial activity Furfurilactobacillus rossiae
1.1.1.28 dimethyl sulfoxide 2%, 88% of initial activity Furfurilactobacillus rossiae
1.1.1.28 Ethanol 2%, 73% of initial activity Furfurilactobacillus rossiae
1.1.1.28 Ethyl acetate 2%, 114% of initial activity Furfurilactobacillus rossiae
1.1.1.28 Glycerol 2%, 92% of initial activity Furfurilactobacillus rossiae
1.1.1.28 isobutanol 2%, 91% of initial activity Furfurilactobacillus rossiae
1.1.1.28 isopropanol 2%, 93% of initial activity Furfurilactobacillus rossiae
1.1.1.28 Methanol 2%, 94% of initial activity Furfurilactobacillus rossiae
1.1.1.28 n-hexane 2%, 80% of initial activity Furfurilactobacillus rossiae

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Furfurilactobacillus rossiae A0A0R1RJ30
-
-
1.1.1.28 Furfurilactobacillus rossiae DSM 15814 A0A0R1RJ30
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 phenylpyruvate + NADH + H+
-
 Furfurilactobacillus rossiae D-phenyllactate + NAD+
-
 ?
1.1.1.28 phenylpyruvate + NADH + H+
-
 Furfurilactobacillus rossiae DSM 15814 D-phenyllactate + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 ? x * 38700, calculated from sequence Furfurilactobacillus rossiae

Synonyms

EC Number Synonyms Comment Organism
1.1.1.28 FD35_GL001981
-
 Furfurilactobacillus rossiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.28 40
-
-
 Furfurilactobacillus rossiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 30
-
 half-life 1453 min Furfurilactobacillus rossiae
1.1.1.28 40
-
 half-life 11 min Furfurilactobacillus rossiae
1.1.1.28 50
-
 half-life 3.7 min Furfurilactobacillus rossiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 12.29
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.28 6.5
-
-
 Furfurilactobacillus rossiae

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.28 6
-
 half-life 895 min Furfurilactobacillus rossiae
1.1.1.28 7
-
 half-life 1655 min Furfurilactobacillus rossiae
1.1.1.28 8
-
 half-life 2319 min Furfurilactobacillus rossiae
1.1.1.28 9
-
 half-life 1315 min Furfurilactobacillus rossiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NADH
-
 Furfurilactobacillus rossiae
1.1.1.28 NADPH NADH is preferred over NADPH Furfurilactobacillus rossiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.28 4.34
-
 phenylpyruvate pH 7, 30°C Furfurilactobacillus rossiae