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Literature summary extracted from

  • Ru, M.; Wang, K.; Bai, Z.; Peng, L.; He, S.; Wang, Y.; Liang, Z.
    A tyrosine aminotransferase involved in rosmarinic acid biosynthesis in Prunella vulgaris L (2017), Sci. Rep., 7, 4892 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expressed in tobacco protoplasts, Escherichia coli Rosetta (DE3) cells and Escherichia coli tyrosine auxotrophic mutant DL39 Prunella vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.4
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.48
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.84
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 1.82
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 3.9
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 4.72
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 8.14
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 10.2
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 10.8
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 52.06
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.5 cytoplasm
-
 Prunella vulgaris 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.5 L-tyrosine + 2-oxoglutarate Prunella vulgaris
-
 4-hydroxyphenylpyruvate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Prunella vulgaris
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 leaf
-
 Prunella vulgaris
-
2.6.1.5 root highest expression Prunella vulgaris
-
2.6.1.5 spike
-
 Prunella vulgaris
-
2.6.1.5 stem
-
 Prunella vulgaris
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 L-glutamate + 4-hydroxyphenylpyruvate
-
 Prunella vulgaris 2-oxoglutarate + L-tyrosine
-
 r
2.6.1.5 L-glutamate + oxaloacetate
-
 Prunella vulgaris ?
-
 r
2.6.1.5 L-glutamate + phenylpyruvate
-
 Prunella vulgaris 2-oxoglutarate + L-phenylalanine
-
 r
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Prunella vulgaris phenylpyruvate + L-glutamate
-
 r
2.6.1.5 L-tryptophan + 2-oxoglutarate
-
 Prunella vulgaris 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + oxaloacetate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-aspartate
-
 r
2.6.1.5 L-tyrosine + phenylpyruvate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-phenylalanine
-
 r
2.6.1.5 L-tyrosine + pyruvate
-
 Prunella vulgaris 4-hydroxyphenylpyruvate + L-alanine
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.5 ? x * 45000, SDS-PAGE Prunella vulgaris

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 TAT
-
 Prunella vulgaris
2.6.1.5 tyrosine aminotransferase
-
 Prunella vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.5 30
-
-
 Prunella vulgaris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 0.11
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.121
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.15
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.3
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.63
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.73
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.89
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 1.05
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 1.19
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 1.55
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8
-
-
 Prunella vulgaris

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.5 Prunella vulgaris calculated from amino acid sequence
-
 6

General Information

EC Number General Information Comment Organism
2.6.1.5 metabolism the enzyme is involved in rosmarinic acid biosynthesis Prunella vulgaris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.5 0.02
-
 pyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.06
-
 oxaloacetate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.06
-
 2-oxoglutarate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.076
-
 phenylpyruvate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.13
-
 L-tryptophan with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.15
-
 L-phenylalanine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.15
-
 oxaloacetate with L-tyrosine as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.18
-
 phenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 0.295
-
 4-hydroxyphenylpyruvate with L-glutamate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris
2.6.1.5 3.03
-
 L-tyrosine with 2-oxoglutarate as cosubstrate, at pH 8.0 and 30°C Prunella vulgaris