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Literature summary extracted from

  • Dow, G.T.; Gilbert, M.; Thoden, J.B.; Holden, H.M.
    Structural investigation on WlaRG from Campylobacter jejuni A sugar aminotransferase (2017), Protein Sci., 26, 586-599 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.B19 purified enzyme in apoform and in complex with dTDP-3-amino-3,6-dideoxy-D-galactose, dTDP-3-acetamido-3,6-dideoxy-D-galactose, and dTDP-3-amino-3,6-dideoxy-D-glucose, hanging drop vapor diffusion method, apoenzyme crystals are grown from 100 mM MOPS, pH 7.0, and 15-18% PEG 3350, at room temperature. Internal aldimine are grown from 100 mM MOPS, pH 7.0, 17-19% PEG 3350, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of dTDP-Qui3N external aldimine complex are grown from 100 mM MOPS, pH 7.0, 12-15% PEG, 10 mM dTDP-Qui3N, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Qui3N are grown from 100 mM HEPES, pH 7.5, 18-21% PEG 5000, 10 mM dTDP-Qui3N, 200 mM KCl, and 1 mM PLP, at room temperature. Crystals of the K184A variant in the presence of dTDP-Fuc3N are grown from 100 mM MOPS, pH 7.0, 13-17% PEG 5000, 10 mM dTDP-Fuc3N, and 1 mM PLP, at room temperature. X-ray diffraction structure determination and analysis at 1.45-1.7 A resolution Campylobacter jejuni
2.6.1.98 purified enzyme in complex with dTDP-3-amino-3,6-dideoxy-D-galactose and dTDP-3-amino-3,6-dideoxy-D-glucose, X-ray diffraction structure determination and analysis at 1.3 A resolution, structure correction Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.B19 K184A site-directed mutagenesis, structure comparison Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.B19 dTDP-3-dehydro-6-deoxy-D-galactose + D-glutamate Campylobacter jejuni
-
 dTDP-3-amino-3,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.B19 dTDP-3-dehydro-6-deoxy-D-glucose + D-glutamate Campylobacter jejuni
-
 dTDP-3-amino-3,6-dideoxy-D-glucose + 2-oxoglutarate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa ATCC 15692
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa 1C
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa PRS 101
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa DSM 22644
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa CIP 104116
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa LMG 12228
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate Pseudomonas aeruginosa JCM 14847
-
 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B19 Campylobacter jejuni Q9ALS9 Penner serotype HS
-
2.6.1.98 Pseudomonas aeruginosa Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa 1C Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa ATCC 15692 Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa CIP 104116 Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa DSM 22644 Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa JCM 14847 Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa LMG 12228 Q9HZ76
-
-
2.6.1.98 Pseudomonas aeruginosa PRS 101 Q9HZ76
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B19 dTDP-3-dehydro-6-deoxy-D-galactose + D-glutamate
-
 Campylobacter jejuni dTDP-3-amino-3,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.B19 dTDP-3-dehydro-6-deoxy-D-glucose + D-glutamate
-
 Campylobacter jejuni dTDP-3-amino-3,6-dideoxy-D-glucose + 2-oxoglutarate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa ATCC 15692 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa 1C UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa PRS 101 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa DSM 22644 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa CIP 104116 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa LMG 12228 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?
2.6.1.98 UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronate + 2-oxoglutarate
-
 Pseudomonas aeruginosa JCM 14847 UDP-2-acetamido-2-deoxy-alpha-D-ribo-hex-3-uluronate + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B19 WlaRG
-
 Campylobacter jejuni
2.6.1.98 WpbE
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B19 pyridoxal 5'-phosphate
-
 Campylobacter jejuni
2.6.1.98 pyridoxal 5'-phosphate
-
 Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
2.6.1.B19 additional information analysis of external aldimine structures of WlaRG solved in the presence of either dTDP-3-amino-3,6-dideoxy-D-galactose or dTDP-3-amino-3,6-dideoxy-D-glucose Campylobacter jejuni
2.6.1.B19 physiological function Campylobacter jejuni 81116 (Penner serotype HS:6) lipoglycan contains two dideoxyhexosamine residues, and this strain can synthesize both dTDP-3-acetamido-3,6-dideoxy-D-glucose and dTDP-3-acetamido-3,6-dideoxy-D-galactose involving enzyme WlaRG, which plays a key role in the production of these unusual sugars by functioning as a pyridoxal 5'-phosphate dependent aminotransferase activity Campylobacter jejuni
2.6.1.98 metabolism the enzyme catalyzes the third step in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid Pseudomonas aeruginosa
2.6.1.98 additional information analysis of external aldimine structures of WpbE solved in the presence of either dTDP-3-amino-3,6-dideoxy-D-galactose or dTDP-3-amino-3,6-dideoxy-D-glucose Pseudomonas aeruginosa
2.6.1.98 physiological function WbpE from Pseudomonas aeruginosa catalyzes the amination of UDP-3-keto-2-acetamido-2-deoxy-D-glucuronic acid (UDP-3-keto-GlcNAcA) representing the third step in the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid Pseudomonas aeruginosa